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Paper Information

Journal:   IRANIAN JOURNAL OF PHARMACEUTICAL RESEARCH (IJPR)   2014 , Volume 13 , Number 4; Page(s) 1437 To 1445.
 
Paper: 

DETERMINATION OF SOME B VITAMINS IN SOUR CHERRY JUICE USING DISPERSIVE LIQUID-LIQUID MICROEXTRACTION FOLLOWED BY HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY

 
 
Author(s):  PARSAEI PARVIN*, BAHMAEI MANOUCHEHR, GHANNADI ALIREZA
 
* DEPARTMENT OF CHEMISTRY, ISLAMIC AZAD UNIVERSITY, NORTH TEHRAN BRANCH, TEHRAN, IRAN
 
Abstract: 

Dispersive liquid-liquid microextraction method (DLLME) combined with high-performance liquid chromatography-ultraviolet detection (HPLC-UV) was used to determine thiamine (B1), nicotinamide (B3) and pyridoxine (B6) in sour cherry juice. This method was rapid, simple and sensitive. Separation was accomplished using a C18 column. The optimum chromatographic conditions were found to be: mobile phase consisted of 8% methanol and 92% aqueous phase (1% (V/V) acetic acid water solution); flow rate, 0.7 mL/min; detection wavelength, 260 nm and pH, 3.3. The extraction efficiency of thiamine, nicotinamide and pyridoxine was influenced by factors such as: additional salt effect, the kind and volume of disperser and extraction solvents. In this research, the limit of detection (LOD) and quantification (LOQ) were 0.9 and 3 ng/mL for thiamine, 1.5 and 5 ng/mL for nicotinamide, 0.9 and 3 ng/mL for pyridoxine. The relative standard deviations (RSDs) were less than 2.87% (n=3). An appropriate linear behavior over the observed concentration range was obtained with the value of R2>0.996 for the target vitamins. This method was successfully applied to the sour cherry juice samples. Sour cherry var. Gise (Prunus cerasus var. Gise), which was used in this research, was a local variety of the sour cherry with large stone, double flowers, double fruits, dark red skin and dark red juice. This variety was identified in high altitude areas of Isfahan province after five years of study, since 2005, by Agricultural and Natural Resources Research Center of Isfahan.

 
Keyword(s): DISPERSIVE LIQUID-LIQUID MICROEXTRACTION (DLLME), HIGH PERFORMANCE LIQUID CHROMATOGRAPHY-ULTRAVIOLET DETECTOR (HPLC-UV), THIAMINE, NICOTINAMIDE, PYRIDOXINE
 
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