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Paper Information

Journal:   IRANIAN JOURNAL OF PHARMACEUTICAL RESEARCH (IJPR)   2014 , Volume 13 , Number 4; Page(s) 1387 To 1392.
 
Paper: 

MCL-1 IS UP REGULATED BY PRENYLATED COUMARIN, UMBELLIPRENIN IN JURKAT CELLS

 
 
Author(s):  GHOLAMI OMID, JEDDI TEHRANI MAHMOOD, IRANSHAHI MEHRDAD, ZARNANI AMIR HASSAN, ZIAI SEYED ALI*
 
* PHARMACOLOGY DEPARTMENT, FACULTY OF MEDICINE, SHAHID BEHESHTI UNIVERSITY OF MEDICAL SCIENCES, TEHRAN, IRAN
 
Abstract: 

Chronic lymphocytic leukaemia (CLL) is the most common B-cell malignancy in the western world and exists as subtypes with very different clinical courses. Myeloid cell leukemia 1 (Mcl-1) is one member of Bcl-2 family proteins that has been shown to be expressed in various tissues and malignant cells, including CLL, where its expression is significantly associated with a failure to achieve complete remission following cytotoxic therapy.
Induction of apoptosis by prenylated coumarin, umbelliprenin, in Jurkat cells was previously shown. We examined whether umbelliprenin can down-regulate Mcl-1 gene and protein in Jurkat cells. In this regard cells were incubated by umbelliprenin, and then down- regulation of Mcl-1 gene was studied by Real Time PCR method. Moreover, down-regulation of Mcl-1 protein was studied by western blot analysis.
We showed that, expression of Mcl-1 mRNA was increased from 1 hour to 3 hours incubation? but this increase has a scale down pattern. Moreover umbelliprenin could inhibit Mcl-1 protein. In conclusion umbelliprenin treatment modulates Mcl-1 expression at both the transcriptional and posttranslational levels.

 
Keyword(s): UMBELLPRENIN, JURKAT CELL, APOPTOSIS, MCL-1, REAL TIME PCR
 
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