Simultaneous application of arbuscular mycorrhizal fungi and organic matter affects the growth and yield of plants. A pot experiment was conducted to study the effects of Funneliformis mosseaefungus, Licorice pulp and their simultaneous application on the morphological and physiological characteristics of pot marigold (Calendula officinalis L.). A completely randomized design with a factorial arrangement in three replications was used. Factors were included 1) arbuscular mycorrhizal fungus, (Funneliformis mosseae and control), 2) four levels of Licorice (0, 5, 10 and 20% (v/v)). Inoculation of pot marigold roots with arbuscular mycorrhizal fungus significantly increased plant height, leaf area, shoot dry weight, chlorophyll and flavonoid content, root colonization, and N, P and K uptake of plant. Application of 5 and 10% v/v of Licorice pulp increased plant height, leaf area, shoot dry weight, flavonoid content, and N, P and K uptake of plant. Flavonoid content increased up to 10% v/v of Licorice pulp application. All measured traits except chlorophyll content, root colonization and flavonoid content significantly decreased at the maximum level of Licorice pulp application (20% v/v). Effect of fungus on all measured traits except flavonoid content was higher than Licorice pulp application. A synergistic effect was observed in co-application treatments of Licorice pulp and Funneliformis mosseae. The positive effects of fungus were higher in 10 and 20% v/v levels of Licorice pulp application for measured parameters.