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Paper Information

Journal:   IRANIAN JOURNAL OF PHARMACEUTICAL RESEARCH (IJPR)   2014 , Volume 13 , Number 4; Page(s) 1447 To 1456.
 
Paper: 

IN-VITRO CALLUS INDUCTION AND ROSMARINIC ACID QUANTIFICATION IN CALLUS CULTURE OF SATUREJA KHUZISTANICA JAMZAD (LAMIACEAE)

 
 
Author(s):  SAHRAROO AMIR, BABALAR MESBAH*, MIRJALILI MOHAMMAD HOSSEIN*, FATTAHI MOGHADDAM MOHAMMAD REZA, NEJAD EBRAHIMI SAMAD
 
* DEPARTMENT OF AGRICULTURE, MEDICINAL PLANTS AND DRUGS RESEARCH INSTITUTE, SHAHID BEHESHTI UNIVERSITY, G. C., EVIN, TEHRAN IRAN
 
Abstract: 

In the present study, an efficient protocol has been developed for callus induction and production of RA in callus culture of Satureja khuzistanica for the first time. In-vitro callus induction was achieved from young shoot tip explants cultured on MS and B5 media supplemented with different concentrations of IBA (0.1, 1.0, 2.0 and 5.0 mgL-1) solely or in combination with cytokinins BAP and KIN (1.0, 2.0 and 5.0 mgL-1). B5 medium supplemented with 1.0 mgL-1 IBA plus 5.0 mgL-1 BAP and MS medium fortified with 2.0 mgL-1 IBA and 2.0 mgL-1 BAP were the most favorable media for callus formation with the highest induction rate (96%). Maximum growth index (2.89 and 2.63) and maximum callus biomass (2.34 and 2.33 g fresh weight) were obtained from the callus cultured on B5 medium supplemented with 1.0 mgL-1 IBA plus 5.0 mgL-1 BAP and MS medium fortified with 1.0 mgL-1 IBA plus 1.0 mgL-1 KIN, respectively. Determination and quantification of RA in cultured calli were performed by HPLC UV/MS analysis. Calli induced from the plant and maintained on supplements of IBA and BAP in the absence of light produced RA 7.5% based on dry weight (DW). No differentiation was observed in any callus during the course of this study.

 
Keyword(s): SATUREJA SP, LAMIACEAE, CALLUS CULTURE, ROSMARINIC ACID, HPLC UV/MS
 
 
References: 
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+ Click to Cite.
APA: Copy

SAHRAROO, A., & BABALAR, M., & MIRJALILI, M., & FATTAHI MOGHADDAM, M., & NEJAD EBRAHIMI, S. (2014). IN-VITRO CALLUS INDUCTION AND ROSMARINIC ACID QUANTIFICATION IN CALLUS CULTURE OF SATUREJA KHUZISTANICA JAMZAD (LAMIACEAE). IRANIAN JOURNAL OF PHARMACEUTICAL RESEARCH (IJPR), 13(4), 1447-1456. https://www.sid.ir/en/journal/ViewPaper.aspx?id=409691



Vancouver: Copy

SAHRAROO AMIR, BABALAR MESBAH, MIRJALILI MOHAMMAD HOSSEIN, FATTAHI MOGHADDAM MOHAMMAD REZA, NEJAD EBRAHIMI SAMAD. IN-VITRO CALLUS INDUCTION AND ROSMARINIC ACID QUANTIFICATION IN CALLUS CULTURE OF SATUREJA KHUZISTANICA JAMZAD (LAMIACEAE). IRANIAN JOURNAL OF PHARMACEUTICAL RESEARCH (IJPR). 2014 [cited 2021November30];13(4):1447-1456. Available from: https://www.sid.ir/en/journal/ViewPaper.aspx?id=409691



IEEE: Copy

SAHRAROO, A., BABALAR, M., MIRJALILI, M., FATTAHI MOGHADDAM, M., NEJAD EBRAHIMI, S., 2014. IN-VITRO CALLUS INDUCTION AND ROSMARINIC ACID QUANTIFICATION IN CALLUS CULTURE OF SATUREJA KHUZISTANICA JAMZAD (LAMIACEAE). IRANIAN JOURNAL OF PHARMACEUTICAL RESEARCH (IJPR), [online] 13(4), pp.1447-1456. Available: https://www.sid.ir/en/journal/ViewPaper.aspx?id=409691.



 
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