Click for new scientific resources and news about Corona[COVID-19]

Paper Information

Journal:   IRANIAN JOURNAL OF PHARMACEUTICAL RESEARCH (IJPR)   2014 , Volume 13 , Number 4; Page(s) 1447 To 1456.
 
Paper: 

IN-VITRO CALLUS INDUCTION AND ROSMARINIC ACID QUANTIFICATION IN CALLUS CULTURE OF SATUREJA KHUZISTANICA JAMZAD (LAMIACEAE)

 
 
Author(s):  SAHRAROO AMIR, BABALAR MESBAH*, MIRJALILI MOHAMMAD HOSSEIN*, FATTAHI MOGHADDAM MOHAMMAD REZA, NEJAD EBRAHIMI SAMAD
 
* DEPARTMENT OF AGRICULTURE, MEDICINAL PLANTS AND DRUGS RESEARCH INSTITUTE, SHAHID BEHESHTI UNIVERSITY, G. C., EVIN, TEHRAN IRAN
 
Abstract: 

In the present study, an efficient protocol has been developed for callus induction and production of RA in callus culture of Satureja khuzistanica for the first time. In-vitro callus induction was achieved from young shoot tip explants cultured on MS and B5 media supplemented with different concentrations of IBA (0.1, 1.0, 2.0 and 5.0 mgL-1) solely or in combination with cytokinins BAP and KIN (1.0, 2.0 and 5.0 mgL-1). B5 medium supplemented with 1.0 mgL-1 IBA plus 5.0 mgL-1 BAP and MS medium fortified with 2.0 mgL-1 IBA and 2.0 mgL-1 BAP were the most favorable media for callus formation with the highest induction rate (96%). Maximum growth index (2.89 and 2.63) and maximum callus biomass (2.34 and 2.33 g fresh weight) were obtained from the callus cultured on B5 medium supplemented with 1.0 mgL-1 IBA plus 5.0 mgL-1 BAP and MS medium fortified with 1.0 mgL-1 IBA plus 1.0 mgL-1 KIN, respectively. Determination and quantification of RA in cultured calli were performed by HPLC UV/MS analysis. Calli induced from the plant and maintained on supplements of IBA and BAP in the absence of light produced RA 7.5% based on dry weight (DW). No differentiation was observed in any callus during the course of this study.

 
Keyword(s): SATUREJA SP, LAMIACEAE, CALLUS CULTURE, ROSMARINIC ACID, HPLC UV/MS
 
References: 
  • ندارد
 
  pdf-File tarjomyar Yearly Visit 66
 
Latest on Blog
Enter SID Blog