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Paper Information

Journal:   IRANIAN JOURNAL OF PHARMACEUTICAL RESEARCH (IJPR)   2014 , Volume 13 , Number 4; Page(s) 1141 To 1148.
 
Paper: 

EFFECT OF ZIRCONIUM DIOXIDE NANOPARTICLES ON GLUTATHIONE PEROXIDASE ENZYME IN PC12 AND N2A CELL LINES

 
 
Author(s):  ASADPOUR ELHAM, SADEGHNIA HAMID REZA, GHORBANI AHMAD, BOROUSHAKI MOHAMMAD TAHER*
 
* DEPARTMENT OF PHARMACOLOGY, FACULTY OF MEDICINE, MASHHAD UNIVERSITY OF MEDICAL SCIENCES, MASHHAD, IRAN
 
Abstract: 

Today, special attention is paid to the use of zirconium dioxide nanoparticle (nano-ZrO2), a neutral bioceramic metal, particularly for drug and gene delivery in medicine. However, there are some reports implying that use of nano-ZrO2 is associated with cytotoxic effects like inhibiting the cell proliferation, DNA damage and apoptosis. In the present study, we examined whether nano-ZrO2 alters cell viability and glutathione peroxidase (GPx) activity in two neuronal cell lines.
The PC12 and N2a cells were cultured in the absence or presence of varying concentrations (31.25-2000
mg/mL) of nano-ZrO2 for 12, 24 or 48 h. The cell viability was evaluated using 3- (4, 5-dimethylthiazol-2-yl) -5- (3-carboxymethoxyphenyl) -2- (4-sulfophenyl) -2H-tetrazolium (MTS) assay and GPx activity was determined by quantifying the rate of oxidation of the reduced glutathione to the oxidized glutathione.
Nano-ZrO2 caused a significant reduction in cell viability and GPx activity after 12, 24 and48 h, as compared with control group. These effects were concentration dependent and started from 250
mg/mL.
The present study demonstrated that nano-ZrO2, at concentrations of>250
mg/mL, has antiproliferative effects via reducing the cell defense mechanism against oxidative stress.

 
Keyword(s): ZIRCONIUM DIOXIDE NANOPARTICLES, PC12 CELL LINE, N2A CELL LINE, OXIDATIVE STRESS, GLUTATHIONE PEROXIDASE ENZYME
 
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