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Paper Information

Journal:   IRANIAN JOURNAL OF PHARMACEUTICAL RESEARCH (IJPR)   2014 , Volume 13 , Number 4; Page(s) 1141 To 1148.
 
Paper: 

EFFECT OF ZIRCONIUM DIOXIDE NANOPARTICLES ON GLUTATHIONE PEROXIDASE ENZYME IN PC12 AND N2A CELL LINES

 
 
Author(s):  ASADPOUR ELHAM, SADEGHNIA HAMID REZA, GHORBANI AHMAD, BOROUSHAKI MOHAMMAD TAHER*
 
* DEPARTMENT OF PHARMACOLOGY, FACULTY OF MEDICINE, MASHHAD UNIVERSITY OF MEDICAL SCIENCES, MASHHAD, IRAN
 
Abstract: 
Today, special attention is paid to the use of zirconium dioxide nanoparticle (nano-ZrO2), a neutral bioceramic metal, particularly for drug and gene delivery in medicine. However, there are some reports implying that use of nano-ZrO2 is associated with cytotoxic effects like inhibiting the cell proliferation, DNA damage and apoptosis. In the present study, we examined whether nano-ZrO2 alters cell viability and glutathione peroxidase (GPx) activity in two neuronal cell lines.The PC12 and N2a cells were cultured in the absence or presence of varying concentrations (31.25-2000 mg/mL) of nano-ZrO2 for 12, 24 or 48 h. The cell viability was evaluated using 3- (4, 5-dimethylthiazol-2-yl) -5- (3-carboxymethoxyphenyl) -2- (4-sulfophenyl) -2H-tetrazolium (MTS) assay and GPx activity was determined by quantifying the rate of oxidation of the reduced glutathione to the oxidized glutathione.Nano-ZrO2 caused a significant reduction in cell viability and GPx activity after 12, 24 and48 h, as compared with control group. These effects were concentration dependent and started from 250 mg/mL.The present study demonstrated that nano-ZrO2, at concentrations of>250 mg/mL, has antiproliferative effects via reducing the cell defense mechanism against oxidative stress.
 
Keyword(s): ZIRCONIUM DIOXIDE NANOPARTICLES, PC12 CELL LINE, N2A CELL LINE, OXIDATIVE STRESS, GLUTATHIONE PEROXIDASE ENZYME
 
 
References: 
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+ Click to Cite.
APA: Copy

ASADPOUR, E., & SADEGHNIA, H., & GHORBANI, A., & BOROUSHAKI, M. (2014). EFFECT OF ZIRCONIUM DIOXIDE NANOPARTICLES ON GLUTATHIONE PEROXIDASE ENZYME IN PC12 AND N2A CELL LINES. IRANIAN JOURNAL OF PHARMACEUTICAL RESEARCH (IJPR), 13(4), 1141-1148. https://www.sid.ir/en/journal/ViewPaper.aspx?id=409689



Vancouver: Copy

ASADPOUR ELHAM, SADEGHNIA HAMID REZA, GHORBANI AHMAD, BOROUSHAKI MOHAMMAD TAHER. EFFECT OF ZIRCONIUM DIOXIDE NANOPARTICLES ON GLUTATHIONE PEROXIDASE ENZYME IN PC12 AND N2A CELL LINES. IRANIAN JOURNAL OF PHARMACEUTICAL RESEARCH (IJPR). 2014 [cited 2021December06];13(4):1141-1148. Available from: https://www.sid.ir/en/journal/ViewPaper.aspx?id=409689



IEEE: Copy

ASADPOUR, E., SADEGHNIA, H., GHORBANI, A., BOROUSHAKI, M., 2014. EFFECT OF ZIRCONIUM DIOXIDE NANOPARTICLES ON GLUTATHIONE PEROXIDASE ENZYME IN PC12 AND N2A CELL LINES. IRANIAN JOURNAL OF PHARMACEUTICAL RESEARCH (IJPR), [online] 13(4), pp.1141-1148. Available: https://www.sid.ir/en/journal/ViewPaper.aspx?id=409689.



 
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