Paper Information

Journal:   ACTA MEDICA IRANICA   2014 , Volume 52 , Number 10; Page(s) 728 To 733.
 
Paper: 

EVALUATION OF THE EXPRESSION OF P-GLYCOPROTEIN IN PROPOXUR-RESISTANT CACO-2 CELLS

 
 
Author(s):  YAZDIAN SHABNAM, FAHHAM NAJMEH, GHAHREMANI MOHAMMAD HOSSEIN, AFZALI MONIREH, FARSANDAJ NARGES, VATANKHAH MELODY, OSTAD SEYED NASSER*
 
* DEPARTMENT OF TOXICOLOGY AND PHARMACOLOGY, OXICOLOGY AND POISONING RESEARCH CENTER, FACULTY OF PHARMACY, TEHRAN UNIVERSITY OF MEDICAL SCIENCES, TEHRAN, IRAN
 
Abstract: 

There is a great concern about the effect of propoxur, as one of the more common N-methyl carbamate pesticides, on human health due to its extensive use in agricultural and non-agricultural applications. Caco-2 cells became resistant to propoxur, and the resistance was confirmed through MTT assay. Then the cell membrane integrity and P-glycoprotein expression were measured by LDH assay and western blot analysis, respectively and compared to the parent cells. Contrary to what was expected, the expression of P-glycoprotein in propoxur resistant cells was lower than parent cells.
Conclusion: This study indicates that the resistance to propoxur may not be related to P-glycoprotein expression directly, since Pglycoprotein expression has decreased in these cells.

 
Keyword(s): PROPOXUR, TOXICITY, P-GLYCOPROTEIN, RESISTANT
 
References: 
 
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