Paper Information

Journal:   IRANIAN JOURNAL OF PHARMACEUTICAL RESEARCH (IJPR)   2014 , Volume 13 , Number 4; Page(s) 1279 To 1294.
 
Paper: 

THE EFFECTS OF EXTENDING OF CO-PLANARITY IN A SERIES OF STRUCTURALLY RELATIVE POLYPYRIDYL PALLADIUM (II) COMPLEXES ON DNA-BINDING AND CYTOTOXICITY PROPERTIES

 
 
Author(s):  SHAHRAKI SOMAYE, MANSOURI TORSHIZI HASSAN*, SORI NEZAMI ZIBA, GHAHGHAEI AREZOU, YAGHOUBI FATEMEH, DIVSALAR ADELEH, SABOURY ALI AKBAR, SHIRAZI FARSHAD H.
 
* DEPARTMENT OF CHEMISTRY, UNIVERSITY OF SISTAN & BALUCHESTAN, ZAHEDAN, IRAN
 
Abstract: 

In depth interaction studies between calf thymus deoxyribonucleic acid (CT-DNA) and a series of four structurally relative palladium (II) complexes [Pd (en) (HB)] (NO3) 2 (a-d), where en is ethylenediamine and heterocyclic base (HB) is 2, 2›-bipyridine (bpy, a); 1, 10-phenanthroline (phen, b); dipyridoquinoxaline (dpq, c) and dipyridophenazine (dppz, d) (Figure 1), were performed. These studies have been investigated by utilizing the electronic absorption spectroscopy, fluorescence spectra and ethidium bromide (EBr) displacement and gel filtration techniques. a-d complexes cooperatively bind and denature the DNA at low concentrations. Their concentration at midpoint of transition, L1.2, follows the order a>>b>c>d. Also the g, the number of binding sites per 1000 nucleotides, follows the order a>>b~c>d. EBr and Scatchard experiments for a-d complexes suggest efficient intercalative binding affinity to CT-DNA giving the order: d>c>b>a. Several binding and thermodynamic parameters are also described. The biological activity of these cationic and water soluble palladium complexes were tested against chronic myelogenous leukemia cell line, K562. b, c and d complexes show cytotoxic concentration (Cc50) values much lower than cisplatin.

 
Keyword(s): PD (II) COMPLEXES, CYTOTOXICITY, DNA BINDING
 
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