Paper Information

Journal:   IRANIAN JOURNAL OF MEDICINAL AND AROMATIC PLANTS   2014 , Volume 30 , Number 2 (64); Page(s) 199 To 215.
 
Paper: 

NITROGEN EFFECTS ON GROWTH, BIOMASS ALLOCATION, ROOT AND SHOOT ALKALOIDS PRODUCTION OF BLACK HENBANE (HYOSCYAMUS NIGER L.) UNDER WATER DECICI STRESS

 
Author(s):  GHORBANPOUR M.*, MAJNON HOSSIENI N., REZAZADEH SH., OMIDI M., KHAVAZI K., HATAMI M., GHAFARZADEGAN R.
 
* DEPARTMENT OF MEDICINAL PLANTS, FACULTY OF AGRICULTURE AND NATURAL RESOURCES, ARAK UNIVERSITY, ARAK, IRAN
 
Abstract: 

In order to indicate the water deficit stress and nitrogen fertilization effects on alkaloids yield and content of Hyoscyamus niger L. plant parts (root and shoot), this experiment was conducted at the full flowering growth stage in greenhouse conditions. Plants were treated with different nitrogen application (0, 75, 150 and 225 kg/ha N as ammonium nitrate in the form of solution, N0-N3) before the commencement of water deficit stress treatment (30, 60 and 90% depletion of water from field capacity, W1-W3). Extracted alkaloids were analyzed by Gas chromatography /mass spectrometery (GC/MS) analysis using a Younglin Acme 6000 GC system equipped with a flame ionization detector (FID) and HP-5MS capillary column (30 m×0.25 mm, film thickness 0.25 mm).
The identification of alkaloids was based on the comparison of their GC retention time and mass spectra data with their standards substances. Results showed that the highest alkaloid content values in root (HYO: 0.281 %DW; SCO: 0.232 % DW) and shoot (HYO: 0.937%DW; SCO: 0.416%DW) were achieved in plants grown under sever water deficit stress (W3) accompanied with nitrogen supply of 225 kg/h (N3). The maximum and minimum (20.52 and 8.95 mg.plant-1) total alkaloids yield in whole plant were obtained in N2W1 and N3W3 treatments, respectively. The survey results indicated that H. niger in the treatment of moderate water deficit stress (W2) along with 150kg N.h-1(N2) in addition to having a good amount of content and performance of both alkaloids, it also contained the highest level of SCO, indicating alkaloid quality.

 
Keyword(s): HYOSCYAMUS NIGER L., TROPANE ALKALOIDS, HYOSCYAMINE, SCOPOLAMINE, NITROGEN, WATER DEFICIT STRESS
 
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