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Paper Information

Journal:   IRANIAN JOURNAL OF PUBLIC HEALTH   AUGUST 2014 , Volume 43 , Number SUPPLEMENT 2; Page(s) 21 To 21.
 
Paper: 

PERSISTENCE OF MYCOBACTERIUM AVIUM PARATUBERCULOSIS IN LIGHVAN CHEESE AS TRACKED BY PROPIDIUM MONOAZIDE QPCR AND MGIT-MPN

 
 
Author(s):  HANIFIAN SHAHRAM*
 
* DEPARTMENT OF FOOD SCIENCE AND TECHNOLOGY, COLLEGE OF AGRICULTURE, TABRIZ BRANCH, ISLAMIC AZAD UNIVERSITY, TABRIZ, IRAN
 
Abstract: 

Background: The aime of this study was to investigate the behavior of Map in Lighvan cheese with special reference to strains of Map, inoculum level, and storage time.
Methods: One standard and two native strains of Map were inoculated (2 and 4 log cell/ml) to ewe’s raw milk and were used for cheese making. Behavior of Map throughout the manufacture, ripening and storage of Lighvan cheese was tracked using propidium monoazide (PMA) quantitative realtime PCR (qPCR) and MGIT-MPN assay.
Results: PMA-qPCR and culture assay demonstrated comparable outcomes. Based on these results, inoculum level and storage time showed a significant effect on persistency of Map. Furthermore, during the storage period different behavior was observed among the various strains of Map.
Conclusion: Map could survive the 6 months of storage period and Lighvan cheese has potential to support the survival of Map.

 
Keyword(s): MYCOBACTERIUM AVIUM PARA TUBERCULOSIS, PROPIDIUM MONOAZIDE, F57-QUANTITATIVE REAL-TIME PCR, MGIT-MPN
 
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