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Paper Information

Journal:   IRANIAN JOURNAL OF PUBLIC HEALTH   AUGUST 2014 , Volume 43 , Number SUPPLEMENT 2; Page(s) 20 To 20.
 
Paper: 

DISTRIBUTION OF MAIN E. COLI PHYLOGROUPS ENCODING CTX-M ESBL IN FECAL ISOLATES OF POULTRY: A SURVEILLANCE STUDY IN FIVE POULTRY HOUSES IN TEHRAN

 
 
Author(s):  DOREGIRAEE FATEMEH*, ALEBOUYEH MASOUD, NAYYERI BAHAR, ZALI MOHAMMAD REZA
 
* DEPARTMENT OF MICROBIOLOGY AND IMMUNOLOGY, FACULTY OF VETERINARY MEDICINE, TEHRAN UNIVERSITY, TEHRAN, IRAN
 
Abstract: 

Background: Association of avian- E. coli phylogroups with human diseases and role of these strains in dissemination of resistance genes to human ones are of major health concerns. This study was aimed to investigate phylogroup diversity and prevalence of ESBL encoding genes among the avian fecal E. coli isolates in five different poultry houses.
Methods: A total of 500 avian cloacal swab samples from five different poultry houses were collected over a six month period in 2013 in Tehran, Iran. Antimicrobial susceptibility testing was done against 13 antibiotics from 9 different classes according to the last CLSI guideline. ESBLs phenotypes were detected by using ceftazidime and ceftazidime-clavulanic acid disks. The presence and type of blatem, blashv, and blactx-M were determined by PCR and sequencing in these isolates. Diversity of phylogroups A, B1, B2 and D were analyzed among the isolates with confirmed ESBLs phenotype by PCR. The plasmid extracts were screened for carriage of
b-lactamase genes.
Results: A total of 444 E. coli isolates were obtained from the studied samples (88%). Nearly 92.6% displayed MDR phenotype. A frequency of 5.4% (26) was confirmed for the isolates presenting ESBL phenotype, which blaCTX-M, bla TEM and blaSHV. Were detected in 6, 10, and 5 strains, respectively. Coexistence of blaTEM and blaCTX-M (3 isolates) and blaTEM, blaCTX-M, and blaSHV (2 isolates) was also detected in these strains. The phylogrouping results showed prevalence of main phylogroups as follow: D (42.3%, 11), B1 (34.6%, 9), A (15.4%, 4), and B2 (7.7%, 2). Plasmid mediated transmission of the
b-lactamase genes were found in 42% of the strains presenting ESBL phenotypes.
Conclusion: The colonization of the avian intestine with E. coli strains related to phylogroups D, as a source of extra intestinal pathogenic E. coli strains responsible for human diseases, and carriage of
b-lactamase genes in these strains proposed them as a source of pathogenic strains in human food chain.

 
Keyword(s): E. COLI, MULTIDRUG RESISTANCE, PHYLOGENETIC GROUPS, EXTENDED-SPECTRUM ?-LACTAMASE, POULTRY
 
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