Paper Information

Journal:   IRANIAN JOURNAL OF PUBLIC HEALTH   AUGUST 2014 , Volume 43 , Number SUPPLEMENT 2; Page(s) 18 To 18.
 
Paper: 

ISOLATION AND IDENTIFICATION OF BACILLUS SUBTILIS FROM THE SOIL SATURATED SALT LAKE QOM AND THE SAFETY AS FOOD PROBIOTICS

 
 
Author(s):  SOVEIZI NAJMEH*, AKHAVAN SEPAHI ABBAS, PAKZAD PARVIZ
 
* DEPARTMENT OF MICROBIOLOGY, FACULTY OF BIOLOGICAL SCIENCES, NORTH TEHRAN BRANCH, ISLAMIC AZAD UNIVERSITY,TEHRAN, IRAN
 
Abstract: 

Background: Health promoting microorganisms, probiotics, have been recently increasingly included in various food products and proposed for use as a food supplement or as a therapy for several infectious diseases. Probiotics based on Bacillus strains have been increasingly proposed for prophylactic and therapeutic use against several gastro-intestinal diseases. We studied the safety of one Bacillus subtilis that isolate from soil saturated salt lake Qom and after isolation and identification bacteria, we studied the safety of this Bacillus strain.
Methods: In this research, we assessed characteristics that are associated with safety of this Bacillus as a probiotic: Soil sampling was done first and then transferred to laboratory and phenotypic and genotypic tests were performed. After 16S rDNA sequencing, the safety studies were performed in bacteria for using as a probiotic.
Results: 16S rDNA revealed that this bacterium is B. subtilis. The strain tested was found to be sensitive to most of the antibiotics used. The strain was non-hemolytic, negative lecithinase and negative enterotoxin gene. There were no differences in the hematological indexes measured in the blood from control and treated mice. Persistence of spores in the mouse gastrointestinal tract counts spores was no longer within detectable levels after 12 days. The strain was shown to produce surfactin and biofilm. This isolate showed auto aggregation and had hydrophobicity in the xylene, chloroform and ethyl acetate. Spores were shown to be completely resistance to gastrointestinal tract (GIT) conditions and the strain produce antimicrobial activity using a panel of Gram positive and negative bacteria.
Conclusion: The surfactin that produced could facilitate entry into cells by rupturing the phospholipid membrane. Biofilm-producing has been shown to remain bacteria in the GIT. Spores were shown to be completely resistance to exposure GIT. This is important as it ensures that the stated dose actually reaches the region of the GIT where it should exert its effect. Hydrophobicity and autoaggregation of the cell increases the level of adhesion and colonization on tissues. Data showed that the strain tested; there was no indication of pathogen city, infection or toxicity study in mice. An important aspect of safety assessments for potential microbial food supplements is to ensure that no nterotoxins are produced by the bacterium and this bacteria didn'show that and no virulence factors like haemolysis and lecithinase are produced. In conclusion, as potential food supplements B. subtilis appeared to show safety.

 
Keyword(s): TOXICITY, SAFETY, PROBIOTICS, BACILLUS SUBTILIS, FOOD
 
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