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Paper Information

Journal:   IRANIAN JOURNAL OF PUBLIC HEALTH   AUGUST 2014 , Volume 43 , Number SUPPLEMENT 2; Page(s) 18 To 18.
 
Paper: 

COMPARISON BETWEEN GENE CASSETTES ARRAY OF INTEGRON CLASS I IN MULTIDRUG RESISTANCE ACINETOBACTER BAUMANNII AND KLEBSIELLA PNEUMONIAE IN IRAN

 
 
Author(s):  JAPONI NEJAD ALIREZA*, VAZIRI FARZAM, GHAZNAVI RAD EHSANOLLAH
 
* DEPARTMENT OF MYCOBACTERIOLOGY, PASTEUR INSTITUTE OF IRAN, TEHRAN, IRAN
 
Abstract: 

Background: Integrin’s are considered to play a major role in multidrug resistance dissemination among gram-negative bacteria. These are genetic elements capable of integrating and mobilizing individual gene cassettes via a site-specific recombination mechanism. Antibiotic resistance in Acinetobacter baumannii and Klebsiella penumoniae is a major problem in the hospital and outbreaks caused by this organism have been reported frequently. The present study aimed at determining the prevalence of different classes of integrin’s and the characterization of integron class 1 gene cassettes in Iranian A. baumannii and K. penumoniae isolates.
Methods: A total of 63 multidrug resistance A. baumannii and 100 K. penumoniae isolates were collected from clinical specimens in the Vali-Asr hospital in the central province of Iran. The antimicrobial susceptibility for 15 antibiotics was determined by disk diffusion. The presence of different integron classes was investigated by PCR and the size of gene cassettes in class 1 integrons was then determined by PCR as well. Moreover, integron cassette arrays of isolates were delineated by RFLP and sequencing amplicons with different lengths.
Results: 90% of K. pneumoniae and 98.4% A. baumannii isolates carried class 1 integrons, whereas class 2 integrons were found in 2% and 15.9% of K.pneumoniae and A. baumannii isolates, respectively. None of the isolates harbored int3. The length of the amplicons ranged from 1000 bp to 3 kb and 500 bp to 3000 bp in K. pneumoniae and A. baumannii isolates, respectively. Sequencing of integrons of class 1 revealed the presence of many resistance genes such as aadA, aacA, aacC, dfrA, blaGES and blaIMP in A. baumannii and dfrA17, aadA5, aac (6') -Ib-cr in K. pneumoniae. Cassette combination aacC1- orfP-orfP- orfQ-aadA1 and dfrA17-aadA5 were most frequently found in A. baumannii and K. pneumoniae , respectively. We identified a completely new gene cassette, which contained aacA7-qacF-aadA5-blaIMP, this cassette has not been reported previously in A. baumannii.
Conclusion: Data from this study demonstrated that class 1 integrons are highly diverse and are associated with a variety of drug resistance phenotypes and drug resistance genes and Integron carriage was significantly associated with an increase in multi- resistance.

 
Keyword(s): INTEGRON CLASS I, ACINETOBACTER BAUMANNII, K. PNEUMONIAE, GENE CASSETTES
 
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