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Paper Information

Journal:   JOURNAL OF RESEARCH IN MEDICAL SCIENCES (JRMS)   MARCH 2014 , Volume 19 , Number SUPPLEMENT 1; Page(s) 30 To 35.
 
Paper: 

EVALUATION OF THE T HELPER 17 CELL SPECIFIC GENES AND THE INNATE LYMPHOID CELLS COUNTS IN THE PERIPHERAL BLOOD OF PATIENTS WITH THE COMMON VARIABLE IMMUNODEFICIENCY

 
 
Author(s):  GANJALIKHANI HAKEMI MAZDAK, YAZDANI REZA, SHERKAT ROYA*, HOMAYOUNI VIDA, MASJEDI MOHSEN, HOSSEINI MOHSEN
 
* ACQUIRED IMMUNODEFICIENCY RESEARCH CENTER, ISFAHAN UNIVERSITY OF MEDICAL SCIENCES, ISFAHAN, IRAN
 
Abstract: 

Background: Common variable immunodeficiency (CVID) is characterized by a deficiency in the immune system with a heterogeneous collection of disorders resulting in antibody deficiency and recurrent infections. T helper 17 (Th 17) cells promote B-cell survival and synergize with the B-cell activating factor to induce their differentiation into the plasma cells. A sub-population of innate lymphoid cells (ILCs) also produces interleukin 17 (IL-17). This study aimed to measure the Th 17 specific genes and ILCs counts in the CVID patients in comparison with control subjects.
Materials and Methods: Total messenger ribonucleic acid (mRNA) was extracted from the whole blood samples of 10 CVID patients and 10 healthy individuals. IL-17, retinoic acid receptor-related orphan receptor C2 (RORC2), IL-23R, and IL-9 gene expression were measured using the quantitative reverse transcriptase-polymerase chain reaction. Count of lineage negative/CD127+/CD90+ ILCs in the blood samples was performed by the flow cytometry method.
Results: The transcript levels of IL-17 and RORC2 in CVID patients was strongly lower than control subjects (P=0.049 and P=0.046, respectively), but slight reduction in the IL-23R expression (P=0.252) have seen in the CVID patients. Accordingly, the number of ILCs decreased significantly (P=0.04). Interestingly, IL-9 mRNA level was more significantly in the CVID patients (P=0.001).
Conclusion: The results presented in this study show that the Th 17 cell specific genes expression (as the determiner Th 17 cells) and ILCs (another lymphoid source of IL-17) are decreased in patients with CVID and this could be an explanation for the defect of their humoral immune response. In addition, elevation of the IL-9 gene expression may shed a new light into the way toward the understanding of the mechanism of autoimmunity in the CVID patients.

 
Keyword(s): COMMON VARIABLE IMMUNODEFICIENCY, INNATE LYMPHOID CELLS, INTERLEUKIN 9, INTERLEUKIN 17, INTERLEUKIN 23R, T HELPER 17 CELLS
 
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