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Paper Information

Journal:   IRANIAN JOURNAL OF VETERINARY MEDICINE (INTERNATIONAL JOURNAL OF VETERINARY RESEARCH)   2014 , Volume 8 , Number 2; Page(s) 135 To 142.
 
Paper: 

DEVELOPMENT OF ANTIBODY-BASED MICROARRAY ASSAY FOR QUANTITATIVE DETECTION OF AFLATOXIN B1

 
 
Author(s):  BEIZAEI A., KAMKAR A.*, MISAGHI A.
 
* DEPARTMENT OF FOOD HYGIENE AND QUALITY CONTROL, FACULTY OF VETERINARY MEDICINE, UNIVERSITY OF TEHRAN, TEHRAN, IRAN
 
Abstract: 

BACKGROUND: Aflatoxin B1 (AFB1) is a toxic metabolite produced by Aspergillus species that contaminates a wide range of agricultural products.
OBJECTIVES: This study was designed to develop a rapid and highly sensitive immunoassay method in microarray format for quantitative detection of AFB1 to evaluate the potential of microarray platform for high-throughput screening, which can be beneficial in food and feed industry.
METHODS: Following successful optimization, using an indirect competitive immunoassay in dot blot format, AFB1-bovine serum albumin (AFB1-BSA) conjugate was contact-printed onto 16 isolated subarrays on multi-pad nitrocellulose coated slides; subsequently used in competitive binding assays.
RESULTS: Using the aforementioned assay, AFB1 was determined from 15 pg/g to 3.04 ng/g working range with detection limit (LOD) of 1 pg/g. To evaluate assay performance in real food matrices, the authors spiked wheat samples with different concentration of AFB1. After extraction, working ranges of 0.11-4.15 ng/g with detection limit of 30pg/g was determined. Good recoveries (94±9%) were obtained, demonstrating accurate detection of AFB1 concentrations in wheat samples. Assay procedure completed in 3 hours.
CONCLUSIONS: The results indicated that the proposed developed assay in microarray format could be used for rapid and sensitive detection of AFB1in wheat samples.

 
Keyword(s): AFLATOXIN B1, DOT BLOT, IMMUNOASSAY, MICROARRAY, OPTIMIZATION
 
References: 
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