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Paper Information

Journal:   IRANIAN JOURNAL OF PUBLIC HEALTH   JULY 2014 , Volume 43 , Number 7; Page(s) 994 To 999.
 
Paper: 

ACTIVITY ASSAY OF GLUTATHIONE S-TRANSFERASE (GSTS) ENZYME AS A DIAGNOSTIC BIOMARKER FOR LIVER HYDATID CYST IN VITRO

 
 
Author(s):  MOATAMEDI POUR LILA, FARAHNAK ALI*, MOLAEI RAD MOHAMADBAGHER, GOLMOHAMADI TAGHI, ESHRAGHIAN MOHAMADREZA
 
* DEPT. OF PARASITOLOGY AND MYCOLOGY, SCHOOL OF PUBLIC HEALTH, TEHRAN UNIVERSITY OF MEDICAL SCIENCES, IRAN
 
Abstract: 

Background: The aim of this study was to detect the Glutathione S-Transferase (GST) enzyme activity of healthy / cystic liver as a diagnostic biomarker for hydatidosis. In order to compare with liver tissue, the level of the GSTs enzyme activityof parasite was also determined.
Methods: Parasites were collected from sheep liver tissue with hydatid cysts at a local abattoir and washed with PBS buffer. Collected parasites and liver tissues were sonicated or homogenized respectively. Extract solution samples were centrifuged and stored at - 20oC. GSTenzyme activities were measured in the extract of parasite and liver tissue samples (healthy and infected livers). Protein amounts and protein bands were detected using Bradford and sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) methods respectively. To determine significant difference between two groups, two-samplet -test was performed.
Results: GST specific activities of healthy / infected livers and parasites were estimated 304, 1297 and 146 U/ml/mgrespectively. Significant higherGST specific activities in cystic liver than healthy liver was observed (P<0.05).
T-test analysis showed GST activity of parasite was lower than healthy liver tissue. SDS-PAGE showed GST protein bands with 24 kDa in parasite samples and25 kDa in liver tissues.
Conclusion: GST activity incystic liver tissue could be concerned as a biomarker for hydatid cyst diagnosis with other hydatid disease parameters.

 
Keyword(s): GLUTATHIONE S-TRANSFERASE, HYDATID CYST, PROTOSCOLICES, LIVER, PARASITE
 
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