Paper Information

Journal:   IRANIAN JOURNAL OF VETERINARY MEDICINE (INTERNATIONAL JOURNAL OF VETERINARY RESEARCH)   2014 , Volume 8 , Number 2; Page(s) 111 To 117.
 
Paper:  GHRELIN IMPROVES RAT SPERM KINEMATIC PARAMETERS DURING ABDOMINAL POSITION OF THE TESTIS
 
Author(s):  KHERADMAND A.*
 
* DEPARTMENT OF CLINICAL SCIENCES, SCHOOL OF VETERINARY MEDICINE, LORESTAN UNIVERSITY, KHORRAM ABAD, IRAN
 
Abstract: 

BACKGROUND: Disruption of testicular function and arrest of spermatogenesis are the consequence of cryptorchidism in response to elevated temperature.
OBJECTIVES: This investigation was set to clarify the possible ghrelin efficacy in altering some sperm quality parameters upon experimentally-induced cryptorchidism.
METHODS: Thirty male adult rats were scheduled for the study and were divided into three groups: group 1 was served as control-saline (CS), group 2 was designed as cryptorchidism-saline (CrS), and group 3 was defined as cryptorchidism-ghrelin (CrG). After surgically inducing cryptorchidism in groups 2 and 3, the researchers gave 10 nmol of ghrelin to CrG rats for 7 consecutive days. Five animals in each group were equally killed on days 3 and 7 after operation and their testes were taken for sperm evaluation.
RESULTS: Testicular weight, sperm forward progressive motility (FPM), functional membrane integrity (assessed by HOS-test), and sperm concentration displayed slight changes after heating on day 3. However, abdominal position of the testes for 7 days caused a significant reduction in the percentages of HOS-positive cells (p<0.0001), FPM (p<0.0001), and sperm concentration (p<0.01). Although a 30% decrease occurred in the testicular weight at this point, however, this reduction was not statistically significant.
Interestingly, HOS-test and FPM percentages were significantly higher in the ghrelin-exposed animals compared to the CrS group on day 7 (p<0.05). However, ghrelin treatment had not considerable influence on sperm parameters by day 3. Moreover, sperm concentration and testicular weight did not exhibit any changes either on day 3 or at day 7 upon ghrelin injection (p>0.05).
CONCLUSIONS: Indeed, this function of ghrelin could be attributed to its antioxidant properties and it may be implicated as a potential agent in attenuation of impaired spermatogenesis after cryptorchidism.

 
Keyword(s): CRYPTORCHIDISM, GHRELIN, RAT, SPERM QUALITY
 
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