Paper Information

Journal:   IRANIAN JOURNAL OF VETERINARY MEDICINE (INTERNATIONAL JOURNAL OF VETERINARY RESEARCH)   2014 , Volume 8 , Number 2; Page(s) 73 To 77.
 
Paper: 

PHYLOGENETIC STUDY BASED ON THE PHOSPHOPROTEIN GENE OF IRANIAN NEWCASTLE DISEASE VIRUSES (NDV) ISOLATES, 2010 -2012

 
 
Author(s):  GHALYANCHI LANGROUDI A.*, HOSSEINI H., KARIMI V., MADADGAR O., HASHEMZADEH M., GHAFOURI S.A., BAGHERI GHADIKOLAEI S.S., VAHEDI S.M.
 
* DEPARTMENT OF MICROBIOLOGY AND IMMUNOLOGY, FACULTY OF VETERINARY MEDICINE, UNIVERSITY OF TEHRAN, TEHRAN, IRAN
 
Abstract: 

BACKGROUND: Newcastle disease virus (NDV) is the causative agent of the Newcastle disease (ND), a highly contagious disease in birds that causes significant economic losses to the poultry industry worldwide. ND is endemic in Iran and outbreaks are reported regularly in commercial poultry flocks and different species of birds.
OBJECTIVES: The current study was carried out to characterize NDV based on phosphorprotein (P) gene from recent outbreaks in Iran, 2010-2012.
METHODS: The P gene fragment of NDV isolates of five chickens, 1 ostrich, and 1 Pigeon paramyxovirus-1 was obtained by RT-PCR and sequenced.
RESULTS: Phylogenetic analysis of sequences revealed that chicken and ostrich NDV isolates were closely related and placed in the genotype VII and Pigeon Paramyxovirus-1 was located in the genotype V.
CONCLUSIONS: This is the first report of Phosphoprotein gene sequences of NDV strains isolated in Iran. This study will help us to understand the epidemiology and molecular characteristics of Newcastle disease virus in Iran.

 
Keyword(s): NEWCASTLE DISEASE, PHOSPHOPROTEIN, PHYLOGENETIC STUDY
 
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