Click for new scientific resources and news about Corona[COVID-19]

Paper Information

Journal:   IRANIAN JOURNAL OF PHARMACEUTICAL RESEARCH (IJPR)   2014 , Volume 13 , Number 3; Page(s) 937 To 944.
 
Paper: 

PROGESTERONE AND CILOSTAZOL PROTECT MICE PANCREATIC ISLETS FROM OXIDATIVE STRESS INDUCED BY HYDROGEN PEROXIDE

 
 
Author(s):  AHANGARPOUR AKRAM, HEIDARI HAMID*, MARD SEYYED ALI, HASHEMITABAR MAHMOUD, KHODADADI ALI
 
* CELLULAR AND MOLECULAR RESEARCH CENTER (CMRC), DEPARTMENT OF PHYSIOLOGY, FACULTY OF MEDICAL SCIENCES, AHVAZ JUNDISHAPUR UNIVERSITY OF MEDICAL SCIENCES, AHVAZ, IRAN
 
Abstract: 

Reactive oxygen species and oxidative stress impair b-cell function and reduce insulin secretion. It has been shown that progesterone and cilostazol possess antioxidant properties. The present study was aimed to investigate in-vitro pretreatment effect of progesterone and cilostazol on insulin secretion as well as their protective effects against hydrogen peroxide-induced oxidative stress in pancreatic isolated islets from mouse.
Pancreatic islets were isolated from 84 male NMRI mice (25-30 g) by collagenase digestion method and pretreated for 48 h with cilostazol (10
mM), progesterone (0.5 mM)and glibenclamide (10 mM) in culture medium. Then islets were exposed to hydrogen peroxide (H2O2.500 mM) for 2 h. Next, culture mediums containing glucose concentration of 2.8 mM or 16.7 mM were added to them and incubated in this status for 1 h. At the end, the rate of insulin output from islets, lipid peroxidation and antioxidant enzymes activities in islet tissues were assayed.
Exposure of islets to H2O2, resulted in a significant decrease in insulin secretion, superoxide dismutase and catalase activities (P<0.001). Also islets malondialdehyde levels were increased by H2O2, after addition of 2.8 mM (P<0.05) and 16.7 mM (P<0.001) glucose.48 h pretreatment of islets with cilostazol and progesterone, significantly reverted back this changes (P<0.05).
Results of present study showed that cilostazol and progesterone protect mice pancreaticislets against H2O2-induced oxidative stress. At the end, our results suggested that protective effects of progesterone and cilostazol are mediated by augmentation the antioxidant defence system of islets.

 
Keyword(s): CILOSTAZOL, H2O2, INSULIN, ISLET, PROGESTERONE
 
References: 
  • ندارد
 
  pdf-File tarjomyar Yearly Visit 67
 
Latest on Blog
Enter SID Blog