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Paper Information

Journal:   IRANIAN JOURNAL OF PHARMACEUTICAL RESEARCH (IJPR)   2014 , Volume 13 , Number 3; Page(s) 853 To 862.
 
Paper: 

A RAPID AND SENSITIVE HPLC-FLUORESCENCE METHOD FOR DETERMINATION OF MIRTAZAPINE AND ITS TWO MAJOR METABOLITES IN HUMAN PLASMA

 
 
Author(s):  LAVASANI HODA, GIORGI MARIO, SHEIKHOLESLAMI BEHJAT, HEDAYATI MOHAMMADHASAN, ROUINI MOHAMMAD REZA*
 
* BIOPHARMACEUTICS AND PHARMACOKINETIC DIVISION, DEPARTMENT OF PHARMACEUTICS, FACULTY OF PHARMACY, TEHRAN UNIVERSITY OF MEDICAL SCIENCES, TEHRAN, IRAN
 
Abstract: 

A rapid and sensitive HPLC method has been developed for the quantification of mirtazapine (MRZ), a noradrenergic and specific serotonergic inhibitor antidepressant (NaSSA) and its two major metabolites N-desmethyl mirtazapine (NDM) and 8-hydroxymirtazapine (8-OHM) in human plasma.
The separation was achieved using Chromolith C18 column and a mobile phase of acetonitrile: phosphate buffer (pH = 3, 20:80, v/v) in isocratic mode at a flow rate of 2 mL/min. A fluorescence detector was set at 290 and 350 nm for excitation and emission, respectively. Zolpidem was used as the internal standard. Liquid-liquid extraction was applied for sample clean up. All analytes were eluted in less than 5 minutes with LOQ of 1 ng/mL for MRZ and 2 ng/mL for both NDM and 8-OHM. The developed method was successfully applied to quantify MRZ and its metabolites in plasma of a healthy volunteer.

 
Keyword(s): MIRTAZAPINE, METABOLITES, HPLC, PLASMA, FLUORESCENCE
 
References: 
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