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Paper Information

Journal:   ARCHIVES OF IRANIAN MEDICINE   FEBRUARY 2013 , Volume 16 , Number 2; Page(s) 104 To 108.
 
Paper: 

COMPARING TCR BETA CHAIN VARIABLE GENE PROFILE SKEWNESS BETWEEN CHILDREN WITH TUBERCULOSIS AND BCG-VACCINATED CHILDREN

 
DOI: 

013162/AIM.0011

 
Author(s):  ZHOU JIAN WEI*, KONG CUI, LUO JUNMIN, CAO JINGHE, SHI YANXI
 
* CLINIC LABORATORY, THE AFFILIATED HOSPITAL OF JINING MEDICAL COLLEGE, JINING 272029, SHANDONG PROVINCE, CHINA
 
Abstract: 

Background: We compared the T cell antigen receptor (TCR-BV) gene families of peripheral blood mononuclear cells (PMBC) between children with tuberculosis (TB) and those inoculated with the Bacille Calmette Guerin (BCG) vaccine.
Methods: The total RNA was extracted from PMBC of 15 TB children, 15 BCG-vaccinated children and 15 healthy controls. The RNAs were reverse-transcribed and amplified by polymerase chain reaction (PCR). PCR products were separated on 1.5% agarose gel and analyzed with the Genescan technique.
Results: Some TCR-BV gene families in TB children and BCG-vaccinated children exhibited a blur band in the predicted position on 1.5% agarose gel, some showed a distinct or fainted band. In general, many shared predominant clonal TCR-BV gene families (V
b2, Vb16, Vb21, Vb22) and the restricted-expression families (Vb14 and Vb17). All the gene families of the control children only exhibited blur bands and polyclonal.
Conclusions: The skewed profile of TCR-BV gene families in TB children and BCG-vaccinated children are similar, which may probably explain the protective effects of BCG-vaccine against TB in children.

 
Keyword(s): BACILLE CALMETTE GUERIN, SKEWED PROFILE, TUBERCULOSIS, T CELL ANTIGEN RECEPTOR
 
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