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Paper Information

Journal:   MODERN GENETICS JOURNAL (MGJ)   2014 , Volume 9 , Number 3 (38); Page(s) 313 To 328.
 
Paper: 

CULTURABLE PROKARYOTIC DIVERSITY OF URMIA SALT LAKE

 
 
Author(s):  JOOKAR KASHI F., OWLIA P., AMOOZEGAR M.A.*, YAKHCHALI B.
 
* UNIVERSITY OF TEHRAN, IRAN
 
Abstract: 

Microbial life is present not only in our familiar world but also in extreme environments. Salt lakes with near or at saturating salinity are extreme environments that common all over the world. The study in detail of such environments would permit to determine not only the microbial diversity but also the gene pools and potential use of this information for biotechnological applications. Urmia Salt Lake in the northwestern of Iran is the second saltiest lake in the world and resembles the Great Salt Lake in the western USA.
Water, soil, sediment and salt samples were taken from east and western sites in Urmia Salt Lake in July 2012. Direct plating, dilution plating and long incubation period were used to isolate organisms on MGM, MH, SWN medium. Isolates were taken from the samples by using the conventional culture-dependent methods. Of these, 36 isolates were selected for sequencing and phylogenetic analysis, based on their growth characteristics and colony morphology. Two hundred and twenty-eight of microorganisms were obtained from soil, salt, water and sediment samples collected from the east and western of the lake. Of these, 36 isolates were selected for sequencing and phylogenetic analysis.
Results showed that 36 strains represented 8 species, belonging to 3 generaHalorubrum, Haloarcula, Haloterrigena. As total, bacterial isolates were belonged to Salicola, Pseudomonas. All strains showed 96.5 to 100 % similarity in 16S rDNA sequencing. Of these, 5 strains showed less than 98.7% sequence similarity to the closest known strains and were representatives as new taxa of Urmia Lake.The phylogenetic analysis of sequences of Urmia Lake indicated in overlaps with 16S rDNA sequences from other lakes with similar habitats.

 
Keyword(s): BIODIVERSITY, EXTREME HALOPHILIC MICROORGANISMS, URMIA SALT LAKE, 16S RDNA
 
References: 
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