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Paper Information

Journal:   IRANIAN JOURNAL OF PATHOLOGY (IJP)   SUMMER 2014 , Volume 9 , Number 3; Page(s) 208 To 214.
 
Paper: 

COMPARISON OF POLYMERASE CHAIN REACTION, ZIEHL-NEELSEN STAININGAND HISTOPATHOLOGIC FINDINGS IN FORMALIN-FIXED, PARAFFIN-EMBEDDED TISSUE SPECIMENS FOR DIAGNOSIS OF TUBERCULOSIS

 
 
Author(s):  KHAZAEI SEDIGHEH, IIZADI BABAK*, ZANDIEH ZHALEH, ALVANDIMANESH AZADEH, VAZIRI SIAVASH
 
* MOLECULAR PATHOLOGY RESEARCH CENTER, IMAM REZA HOSPITAL, KERMANSHAH UNIVERSITY OF MEDICAL SCIENCES, KERMANSHAH, IRAN
 
Abstract: 

Background and Objective: Tuberculosis is still a major health problem, involving about 1.3 of the world´s population. Diagnosis is difficult when we only use Ziehl-Neelson staining. Many cases may be missed. A more rapid and sensitive diagnostic method is necessary. PCR may be helpful. The aim of this study is to compare PCR, Zieh-Neelsen staining and histopathologic findings in diagnosis of tuberculosis on formalin-fixed paraffin-embedded tissues.
Methods: Paraffin blocks of the submitted specimens of the patients clinically suspicious for tuberculosis or containing granuloma were selected. Ziehl-Neelsen Staining & TB-PCR (IS6110 element) were carried out. The results of the tests were compared by using the McNemar test. Statistical significance was accepted when the P value was less than 0.05.
Results: Forty five specimens were included in the study, 35 had granulomas (19 with caseous necrosis). Acid-fast bacilli were identified in 17 specimens (37.8%). TB-PCR was positive in 16specimens (84%) with caseating granulomas, 11 specimens (68.8%) with non-caseating granulomas & 6 specimens (60%) without granulomas. (P value=0.59).
Conclusions: TB-PCR on paraffin–embedded tissue is a potentially useful approach for early, rapid and sensitive diagnosis of tuberculosis. It is especially useful when granuloma is seen in tissue section, while acid-fast stain is negative. If there was no facilities for PCR, histopathological diagnosis with clinical correlation is more reliable in comparison to AFB results.

 
Keyword(s): PCR, STAINING, PATHOLOGY, TUBERCULOSIS
 
References: 
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