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Paper Information

Journal:   ADVANCED PHARMACEUTICAL BULLETIN   2013 , Volume 3 , Number 2; Page(s) 333 To 337.
 
Paper: 

A UNIQUE REPORT: DEVELOPMENT OF SUPER ANTI-HUMAN IGG MONOCLONE WITH OPTICAL DENSITY OVER THAN 3

 
 
Author(s):  AGHEBATI MALEKI LEILI, BARADARAN BEHZAD, ABDOLALIZADEH JALAL, EZZATIFAR FATEMEH, MAJIDI JAFAR*
 
* IMMUNOLOGY RESEARCH CENTER, TABRIZ UNIVERSITY OF MEDICAL SCIENCES, TABRIZ, IRAN
 
Abstract: 

Purpose: Monoclonal antibodies and related conjugates are key reagents used in biomedical researches as well as, in treatment, purification and diagnosis of infectious and non- infectious diseases.
Methods: Balb/c mice were immunized with purified human IgG. Spleen cells of the most immune mouse were fused with SP2.0 in the presence of Poly Ethylene Glycol (PEG). Supernatant of hybridoma cells was screened for detection of antibody by ELISA. Then, the sample was assessed for cross-reactivity with IgM & IgA by ELISA and confirmed by immunoblotting. The subclasses of the selected mAbs were determined. The best clone was injected intraperitoneally to some pristane-injected mice. Anti-IgG mAb was purified from the animals' ascitic fluid by Ion exchange chromatography and then, mAb was conjugated with HRP.
Results: In the present study, over than 50 clones were obtained that 1 clone had optical density over than 3. We named this clone as supermonoclone which was selected for limiting dilution. The result of the immunoblotting, showed sharp band in IgG position and did not show any band in IgM& IgA position.
Conclusion: Based on the findings of this study, the conjugated monoclonal antibody could have application in diagnosis of infectious diseases like Toxoplasmosis, Rubella and IgG class of other infectious and non- infectious diseases.

 
Keyword(s): MONOCLONAL ANTIBODY, HUMAN IGG, BALB/C MICE, ELISA
 
 
References: 
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Citations: 
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APA: Copy

AGHEBATI MALEKI, L., & BARADARAN, B., & ABDOLALIZADEH, J., & EZZATIFAR, F., & MAJIDI, J. (2013). A UNIQUE REPORT: DEVELOPMENT OF SUPER ANTI-HUMAN IGG MONOCLONE WITH OPTICAL DENSITY OVER THAN 3. ADVANCED PHARMACEUTICAL BULLETIN, 3(2), 333-337. https://www.sid.ir/en/journal/ViewPaper.aspx?id=408926



Vancouver: Copy

AGHEBATI MALEKI LEILI, BARADARAN BEHZAD, ABDOLALIZADEH JALAL, EZZATIFAR FATEMEH, MAJIDI JAFAR. A UNIQUE REPORT: DEVELOPMENT OF SUPER ANTI-HUMAN IGG MONOCLONE WITH OPTICAL DENSITY OVER THAN 3. ADVANCED PHARMACEUTICAL BULLETIN. 2013 [cited 2021November27];3(2):333-337. Available from: https://www.sid.ir/en/journal/ViewPaper.aspx?id=408926



IEEE: Copy

AGHEBATI MALEKI, L., BARADARAN, B., ABDOLALIZADEH, J., EZZATIFAR, F., MAJIDI, J., 2013. A UNIQUE REPORT: DEVELOPMENT OF SUPER ANTI-HUMAN IGG MONOCLONE WITH OPTICAL DENSITY OVER THAN 3. ADVANCED PHARMACEUTICAL BULLETIN, [online] 3(2), pp.333-337. Available: https://www.sid.ir/en/journal/ViewPaper.aspx?id=408926.



 
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