Paper Information

Journal:   ADVANCED PHARMACEUTICAL BULLETIN   2013 , Volume 3 , Number 2; Page(s) 329 To 332.
 
Paper: 

INDUCTION OF CD14 EXPRESSION AND DIFFERENTIATION TO MONOCYTES OR MATURE MACROPHAGES IN PROMYELOCYTIC CELL LINES: NEW APPROACH

 
 
Author(s):  ZAMANI FATEMEH, ZARE SHAHNEH FATEMEH, AGHEBATI MALEKI LEILI, BARADARAN BEHZAD*
 
* IMMUNOLOGY RESEARCH CENTER, FACULTY OF MEDICINE, TABRIZ UNIVERSITY OF MEDICAL SCIENCES, TABRIZ, IRAN
 
Abstract: 

Purpose: CD14, one of the main differentiation markers on the surface of myeloid lineage cells, acts as a key role in activation of LPS-induced monocytes. LPS (lipopolysaccharide) binds to LPS-binding protein in plasma and are delivered to the cell surface receptor CD14. In this study, Various stimuli [Dimethyl Sulfoxide (DMSO), active 1, 25-dihydroxyvitamin D3 [1, 25 (OH) 2D3] and LPS], either alone or in combination, have been recognized that have an effect on the level of CD14 expression in the human HL-60 and U937 promonocytic cell lines and therefore induce their terminal differentiation into monocytes or mature macrophages.
Methods: U937 and HL-60 cells were cultured in RPMI 1640 supplemented with 10% FBS. For each cell line, 1×106 cells were seeded for 72 hours with DMSO, 14 days with LPS and 18 days with 1, 25-D3 in each well plate; then ELISA method was used to study their responses to the factors by means of anti-CD14.
Results: ELISA assay demonstrated that U937 and HL-60 cells were induced by both [1, 25 (OH) 2D3] and DMSO to obtain characteristics of adherent cells and express CD14 protein; moreover, LPS at a low dose increased CD14 expression on surface of this cells.
Conclusion: According to the our results, it is speculated that CD14 gene expression may be induced in human U937 and HL-60 cell lines by different factors including 1, 25-D3, DMSO and LPS.

 
Keyword(s): CD14, 1, 25-D3, LPS, DMSO, MONOCYTE
 
References: 
  • ندارد
 
  pdf-File tarjomyar Yearly Visit 61
 
Latest on Blog
Enter SID Blog