Paper Information

Journal:   IRANIAN RED CRESCENT MEDICAL JOURNAL (IRCMJ)   MAY 2014 , Volume 16 , Number 5; Page(s) 1 To 6.
 
Paper: 

ANTIBIOTIC SUSCEPTIBILITY PATTERNS AND MOLECULAR EPIDEMIOLOGY OF METALLO-β-LACTAMASE PRODUCING PSEUDOMONAS AERUGINOSA STRAINS ISOLATED FROM BURN PATIENTS

 
 
Author(s):  JAPONI AZIZ, ANVARINEJAD MOJTABA*, FARSHAD SHOHREH, GIAMMANCO GIOVANNI M., RAFAATPOUR NORODDIN, ALIPOUR EBRAHIM
 
* ALBORZI CLINICAL MICROBIOLOGY RESEARCH CENTER, NEMAZEE TEACHING HOSPITAL, SHIRAZ UNIVERSITY OF MEDICAL SCIENCES, SHIRAZ, IR IRAN
 
Abstract: 

Background: Failure in the treatment of burn patients infected with Pseudomonas aeruginosa could happen as a result of the acquisition of antibiotic resistance, including carbapenems.
Objectives: The aim of the present study was to investigate the phenotypic and genotypic characteristics of the Pseudomonas aeruginosa strains, isolated from burn patients.
Patients and Methods: During a 12 month period, in this cross-sectional study, two hundred seventy strains of Pseudomonas aeruginosa were isolated from the burn patients in Ghotbeddin Burn Hospital, Shiraz, Iran. Screening for the carbapenem resistance in the isolates was carried out by the E test method. Sensitivity patterns of metallo-
b-lactamase (MbLs) producing strains of pseudomonas to eleven antibiotics were determined by the mentioned method. The epidemiological associations of these strains were determined by Pulsed-field gel electrophoresis (PFGE).
Results: Of the 270 strains, 60 (22.2%) were resistant to imipenem and meropenem, classified as M
bLs producing. MbLs producing strains of pseudomonas were completely resistant to five tested antibiotics while their sensitivities to the three most effective antibiotics including ceftazidime, amikacin and ciprofloxacin were 23.4%, 6.7 % and 1.7%, respectively. In PFGE, 37 patterns from the genome of Pseudomonas aeruginosa were observed. Majority of the strains (43, 71.6%) exhibited more than 80% similarity, based on the drawn dendrogram.
Conclusions: According to the results, none of the tested antibiotics is safe to prescribe. As PFGE revealed, a limited number of Pseudomonas aeruginosa types are predominant in the hospitals which infect the burn patients.

 
Keyword(s): BETA-LACTAMASES, IMIPENEM, MEROPENEM, PULSED FIELD GEL ELECTROPHORESI
 
References: 
  • ندارد
 
  pdf-File tarjomyar Yearly Visit 69
 
Latest on Blog
Enter SID Blog