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Paper Information

Journal:   ADVANCED PHARMACEUTICAL BULLETIN   2013 , Volume 3 , Number 2; Page(s) 289 To 293.
 
Paper: 

DEVELOPMENT AND APPLICATION OF AN HPLC METHOD FOR ERLOTINIB PROTEIN BINDING STUDIES

 
 
Author(s):  BOLANDNAZAR SOHEILA, DIVSALAR ADELEH, VALIZADEH HADI, KHODAEI ARASH, ZAKERI MILANI PARVIN*
 
* DEPARTMENT OF PHARMACEUTICS, FACULTY OF PHARMACY, TABRIZ UNIVERSITY OF MEDICAL SCIENCES, TABRIZ, IRAN. 51664
 
Abstract: 

Purpose: The aim of the present study was to develop a simple and rapid reversed-phase high performance liquid chromatographic method with UV detection for erlotinib hydrochloride quantification, which is applicable for protein binding studies.
Methods: Ultrafilteration method was used for protein binding study of erlotinib hydrochloride. For sample analysis a simple and rapid reversed-phase high performance liquid chromatographic method with UV detection at 332 nm was developed. The mobile phase was a mixture of methanol, acetonitril and potassium dihydrogen phosphate buffer (15: 45: 40%v/v) set at flow rate of 1.3 ml/min.
Results: The run time for erlotinib hydrochloride was approximately 6 minutes. The calibration curve was linear over the range of 320-20000 ng/ml with acceptable intra- and inter-day precision and accuracy. The intra-day and inter-day precisions were less than 10% and the accuracies of intra and inter-day assays were within the range of 97.20-104.83% and 98.8-102.2% respectively.
Conclusion: Based on the obtained results, a simple, accurate and precise reversed-phase isocratic HPLC method with UV detection has been optimized and validated for the determination of erlotinib hydrochloride in biological samples.

 
Keyword(s): ERLOTINIB, HPLC, PROTEIN BINDING, ULTRAFILTERATION
 
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