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Paper Information

Journal:   NOVELTY IN BIOMEDICINE   2014 , Volume 2 , Number 1; Page(s) 18 To 21.
 
Paper: 

DETECTION OF ACTA AND INLB GENES IN LISTERIA MONOCYTOGENES ISOLATED FROM WOMEN WITH SPONTANEOUS ABORTIONS

 
 
Author(s):  ESLAMI GITA, SAMADI ROGHAYEH*, TAHERPANAH ROBABE, TAHERPOR AREZU, BASERI NEDA
 
* DEPARTMENT OF MICROBIOLOGY, FACULTY OF MEDICINE, SHAHID BEHESHTI UNIVERSITY OF MEDICAL SCIENCES, TEHRAN, IRAN
 
Abstract: 

Background & Objective: Assessment of the extent of the role of L. monocytogenes in human spontaneous abortions, using isolation methods and PCR analysis for the presence of actA and InlB genes.
Methods and Materials: In this descriptive study, vaginal swabs were collected from 96 women with spontaneous abortions who referred to Tehran`s hospitals. The samples were cultured in to Listeria specific media (PALCAM agar). Then, the Listeria genus was verified by differentiation biochemical tests, such as, hemolysis on Blood agar, Catalase and Oxidase reactions, and motility at room temperature. PCR technique was performed on all samples and detected the actA and InlB genes of L. monocytogenes.
Results: In culture, 7 of 96 samples were positive for L.monocytogenes. With the PCR technique, actA and InlB genes were detected from 12 and 2 vaginal samples, respectively.
Conclusions: The occurrence of pathogenic L. monocytogenes in cases of spontaneous abortions was 12.5%. It seems that the actA and InlB genes have important roles as essential virulence factors in pathogenic L.monocytogenes. The results show the PCR method is more sensitive, easier and faster than culture to detect L.monocytogenes.

 
Keyword(s): LISTERIA MONOCYTOGENES, SPONTANEOUS ABORTIONS, ACTA GENE, INLB GENE
 
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