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Paper Information

Journal:   ADVANCED PHARMACEUTICAL BULLETIN   2014 , Volume 4 , Number 3; Page(s) 267 To 272.
 
Paper: 

CHIRAL SEPARATION OF INDAPAMIDE ENANTIOMERS BY CAPILLARY ELECTROPHORESIS

 
 
Author(s):  TERO VESCAN AMELIA, HANCU GABRIEL*, OROIAN MIHAELA, CARJE ANCA
 
* FACULTY OF PHARMACY, UNIVERSITY OF MEDICINE AND PHARMACY TARGU MURES, GHMARINESCU 38, 540000 TARGU MURES, ROMANIA
 
Abstract: 

Purpose: Indapamide is probably the most frequently prescribed diuretic drug, generally being used for the treatment of hypertension. It contains a chiral center in its molecule; is marketed as a racemic mixture; but there are rather few studies regarding the pharmacokinetic and the pharmacological effect differences of the two enantiomers. Our aim was the development of a simple, rapid and precise analytical procedure for the chiral separation of indapamide enantiomers.
Methods: In this study capillary zone electrophoresis was used for the enantiomeric separation of indapamide using a systematic screening approach involving different native and derivatized; neutral and charged cyclodextrines as chiral selectors. The effects of pH value and composition of the background electrolyte, capillary temperature, running voltage and injection parameters have been investigated.
Results: After preliminary analysis a charged derivatized CD, sulfobuthyl ether-
b-CD, proved to be the optimum chiral selector for the enantioseparation. Using a buffer solution containing 25 mM disodium hydrogenophosphate – 25 mM sodium didydrogenophosphate and 5 mM sulfobuthyl ether- b-CD as chiral selector at a pH - 7, a voltage of+25 kV, temperature 15oC and UV detection at 242 nm, we succeeded in the separation of the two enantiomers in approximately 6 minutes, with a resolution of 4.30 and a separation factor of 1.08.
Conclusion: Capillary zone electrophoresis using cyclodextrines as chiral selectors proved to be a suitable method for the enantioseparation of indapamide. Our method is rapid, specific, reliable, and cost-effective and can be proposed for laboratories performing indapamide routine analysis.

 
Keyword(s): INDAPAMIDE, CAPILLARY ELECTROPHORESIS, CHIRAL SEPARATION, CYCLODEXTRINES
 
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