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Paper Information

Journal:   ADVANCED PHARMACEUTICAL BULLETIN   2014 , Volume 4 , Number 3; Page(s) 243 To 248.
 
Paper: 

THERAPEUTIC EFFECTS OF MYELOID CELL LEUKEMIA-1 SIRNA ON HUMAN ACUTE MYELOID LEUKEMIA CELLS

 
 
Author(s):  KARAMI HADI, BARADARAN BEHZAD*, ESFAHANI ALI, SAKHINIA MASOUD, SAKHINIA EBRAHIM*
 
* DEPARTMENT OF GENETICS, FACULTY OF MEDICINE, TABRIZ UNIVERSITY OF MEDICAL SCIENCES, TABRIZ, IRAN
 
Abstract: 

Purpose: Up-regulation of Mcl-1, a known anti-apoptotic protein, is associated with the survival and progression of various malignancies including leukemia. The aim of this study was to explore the effect of Mcl-1 small interference RNA (siRNA) on the proliferation and apoptosis of HL-60 acute myeloid leukemia (AML) cells.
Methods: siRNA transfection was performed using LipofectamineTM 2000 reagent. Relative mRNA and protein expressions were quantified by quantitative real-time PCR and Western blotting, respectively. Trypan blue assay was performed to assess tumor cell proliferation after siRNA transfection. The cytotoxic effect of Mcl-1 siRNA on leukemic cells was measured using MTT assay. Apoptosis was detected using ELISA cell death assay.
Results: Mcl-1 siRNA clearly lowered both Mcl-1 mRNA and protein levels in a time-dependent manner, leading to marked inhibition of cell survival and proliferation. Furthermore, Mcl-1 down-regulation significantly enhanced the extent of HL-60 apoptotic cells.
Conclusion: Our results suggest that the down-regulation of Mcl-1 by siRNA can effectively trigger apoptosis and inhibit the proliferation of leukemic cells. Therefore, Mcl-1 siRNA may be a potent adjuvant in AML therapy.

 
Keyword(s): MCL-1, SIRNA, LEUKEMIA, HL-60, APOPTOSIS, PROLIFERATION
 
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