Click for new scientific resources and news about Corona[COVID-19]

Paper Information

Journal:   JUNDISHAPUR JOURNAL OF MICROBIOLOGY (JJM)   MAY 2014 , Volume 7 , Number 5; Page(s) 0 To 0.
 
Paper: 

DETECTION OF HUMAN PAPILLOMAVIRUS IN CHRONIC CERVICITIS, CERVICAL ADENOCARCINOMA, INTRAEPITHELIAL NEOPLASIA AND SQUAMUS CELL CARCINOMA

 
 
Author(s):  MIRZAIE KASHANI ELAHE, BOUZARI MAJID*, TALEBI ARDESHIR, ARBABZADEH ZAVAREH FARAHNAZ
 
* DEPARTMENT OF BIOLOGY, FACULTY OF SCIENCE, UNIVERSITY OF ISFAHAN, ISFAHAN, IR IRAN
 
Abstract: 

Background: Cervical cancer is the second most common cancer in women worldwide. Recent studies show that human papillomavirus (HPV) DNA is present in all cervical carcinomas and in some cervicitis cases, with some geographical variation in viral subtypes. Therefore determination of the presence of HPV in the general population of each region can help reveal the role of these viruses in tumors.
Objectives: This study aimed to estimate the frequency of infection with HPV in cervicitis, cervical adenocarcinoma, intraepithelial neoplasia and squamus cell carcinoma samples from the Isfahan Province, Iran.
Patients and Methods: One hundred and twenty two formalin fixed paraffin embedded tissue samples of crevicitis cases and different cervix tumors including cervical intraepithelial neoplasia (CIN) (I, II, III), squamus cell carcinoma (SCC) and adenocarcinoma were collected from histopathological files of Al-Zahra Hospital in Isfahan. Data about histopathological changes were collected by reexamination of the hematoxylin and eosin stained sections. DNA was extracted and subjected to Nested PCR using consensus primers, MY09/MY11 and GP5+/GP6+, designed for amplification of a conserved region of the genome coding for L1 protein.
Results: In total 74.5% of the tested samples were positive for HPV. Amongst the tested tumors 8 out of 20 (40%) of CIN (I, II, III), 5 out of 21 (23.8%) of adenocarcinoma cases and 78 out of 79 chronic cervicitis cases were positive for HPV.
Conclusions: The rate of different carcinomas and also the rate of HPV infection in each case were lower than other reports from different countries. This could be correlated with the social behavior of women in the area, where they mostly have only one partner throughout their life, and also the rate of smoking behavior of women in the studied population. On the other hand the rate of HPV infection in chronic cervicitis cases was much higher than cases reported by previous studies. This necessitates more attention to the role of human papillomaviruses in the their induction in the studied area.

 
Keyword(s): POLYMERASE CHAIN REACTION, UTERINE CERVICAL NEOPLASMS, UTERINE CERVICITIS
 
 
References: 
  • ندارد
  •  
 
Click to Cite.
APA: Copy

MIRZAIE KASHANI, E., & BOUZARI, M., & TALEBI, A., & ARBABZADEH ZAVAREH, F. (2014). DETECTION OF HUMAN PAPILLOMAVIRUS IN CHRONIC CERVICITIS, CERVICAL ADENOCARCINOMA, INTRAEPITHELIAL NEOPLASIA AND SQUAMUS CELL CARCINOMA. JUNDISHAPUR JOURNAL OF MICROBIOLOGY (JJM), 7(5), 0-0. https://www.sid.ir/en/journal/ViewPaper.aspx?id=408411



Vancouver: Copy

MIRZAIE KASHANI ELAHE, BOUZARI MAJID, TALEBI ARDESHIR, ARBABZADEH ZAVAREH FARAHNAZ. DETECTION OF HUMAN PAPILLOMAVIRUS IN CHRONIC CERVICITIS, CERVICAL ADENOCARCINOMA, INTRAEPITHELIAL NEOPLASIA AND SQUAMUS CELL CARCINOMA. JUNDISHAPUR JOURNAL OF MICROBIOLOGY (JJM). 2014 [cited 2021May09];7(5):0-0. Available from: https://www.sid.ir/en/journal/ViewPaper.aspx?id=408411



IEEE: Copy

MIRZAIE KASHANI, E., BOUZARI, M., TALEBI, A., ARBABZADEH ZAVAREH, F., 2014. DETECTION OF HUMAN PAPILLOMAVIRUS IN CHRONIC CERVICITIS, CERVICAL ADENOCARCINOMA, INTRAEPITHELIAL NEOPLASIA AND SQUAMUS CELL CARCINOMA. JUNDISHAPUR JOURNAL OF MICROBIOLOGY (JJM), [online] 7(5), pp.0-0. Available: https://www.sid.ir/en/journal/ViewPaper.aspx?id=408411.



 
  pdf-File
Yearly Visit 83
 
Latest on Blog
Enter SID Blog