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Paper Information

Journal:   JUNDISHAPUR JOURNAL OF MICROBIOLOGY (JJM)   AUGUST 2014 , Volume 7 , Number 8; Page(s) 0 To 0.
 
Paper: 

PREVALENCE AND CHARACTERIZATION OF CLOSTRIDIUM DIFFICILE IN BEEF AND MUTTON MEATS OF ISFAHAN REGION, IRAN

 
 
Author(s):  ESFANDIARI ZAHRA, JALALI MOHAMMAD, EZZATPANAH HAMID*, WEESE J.SCOTT, CHAMANI MOHAMMAD
 
* DEPARTMENT OF FOOD SCIENCE AND TECHNOLOGY, COLLEGE OF FOOD SCIENCE AND TECHNOLOGY, TEHRAN SCIENCE AND RESEARCH BRANCH, ISLAMIC AZAD UNIVERSITY, TEHRAN, 14515.775, IR IRAN
 
Abstract: 

Background: Clostridium difficile (C. difficile) is a frequent cause of nosocomial infections. During last few years, the mortality rate of C. difficile infection (CDI) increased in healthcare facilities. This organism has become a major public health concern in developed nations. Because of the increasing incidence of acquired-CDI (CA-CDI) and notable genetic overlap between C. difficile isolates from animals and humans, meat has defined as one of the probable transmission route of C. diffiicle to humans.
Objectives: This study was performed to determine the prevalence of toxigenic C. difficile in beef and mutton meats consumed as human food in Isfahan, central part of Iran. Furthermore the polymerase chain reaction (PCR) -ribotyping employed to compare the genetic pattern of positive isolates in meat with clinical ones.
Materials and Methods: A total of 200 raw meat samples (81 beef and 119 mutton) were purchased from meat packaging plants. The samples were anaerobically cultured in C. difficile moxalactam norfloxacin (CDMN) broth and plated on selective enrichment medium. The suspicious colonies were recultured on blood agar anaerobically. All C. difficile isolates identified by morphological and biochemical testing were screened by PCR for the presence of genes encoding the triose phosphate isomerase (tpi), toxin A (tcdA), toxin B (tcdB) and binary toxin (cdtB). The genomes of extracted isolates were analyzed by 16S-23S rRNA-based PCR ribotyping.
Results: The overall prevalence of C. difficile with two toxigenic genes including tcdA and tcdB was estimated at 4.0%. C. difficile was detected in 2.8%, 2.1%, 3.6% and 6.2% of chopped beef, ground beef, chopped mutton and ground mutton, respectively. The cdtB gene was not found in positive isolates. Eight different ribotypes were found in isolated strains that were not identical with those belonging to patients with CDI.
Conclusions: The results of PCR-ribotyping indicate that no relationship exists between clinical and meat isolates. We therefore conclude that other sources than meat may function as a vector for CA-CDI.

 
Keyword(s): CLOSTRIDIUM DIFFICILE, MEAT, PCR, IRAN
 
 
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APA: Copy

ESFANDIARI, Z., & JALALI, M., & EZZATPANAH, H., & WEESE, J., & CHAMANI, M. (2014). PREVALENCE AND CHARACTERIZATION OF CLOSTRIDIUM DIFFICILE IN BEEF AND MUTTON MEATS OF ISFAHAN REGION, IRAN. JUNDISHAPUR JOURNAL OF MICROBIOLOGY (JJM), 7(8), 0-0. https://www.sid.ir/en/journal/ViewPaper.aspx?id=408362



Vancouver: Copy

ESFANDIARI ZAHRA, JALALI MOHAMMAD, EZZATPANAH HAMID, WEESE J.SCOTT, CHAMANI MOHAMMAD. PREVALENCE AND CHARACTERIZATION OF CLOSTRIDIUM DIFFICILE IN BEEF AND MUTTON MEATS OF ISFAHAN REGION, IRAN. JUNDISHAPUR JOURNAL OF MICROBIOLOGY (JJM). 2014 [cited 2021May12];7(8):0-0. Available from: https://www.sid.ir/en/journal/ViewPaper.aspx?id=408362



IEEE: Copy

ESFANDIARI, Z., JALALI, M., EZZATPANAH, H., WEESE, J., CHAMANI, M., 2014. PREVALENCE AND CHARACTERIZATION OF CLOSTRIDIUM DIFFICILE IN BEEF AND MUTTON MEATS OF ISFAHAN REGION, IRAN. JUNDISHAPUR JOURNAL OF MICROBIOLOGY (JJM), [online] 7(8), pp.0-0. Available: https://www.sid.ir/en/journal/ViewPaper.aspx?id=408362.



 
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