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Paper Information

Journal:   ADVANCED PHARMACEUTICAL BULLETIN   2014 , Volume 4 , Number 1; Page(s) 61 To 67.
 
Paper: 

ANALYSIS OF CAROTENOID PRODUCTION BY HALORUBRUM SP. TBZ126, AN EXTREMELY HALOPHILIC ARCHEON FROM URMIA LAKE

 
 
Author(s):  NAZIRI DAVOOD, HAMIDI MASOUD, HASSANZADEH SALAR, TARHRIZ VAHIDEH, MALEKI ZANJANI BAHRAM, NAZEMYIEH HOSSEIN, HEJAZI MOHAMMD AMIN*, HEJAZI MOHAMMAD SAEID*
 
* FACULTY OF PHARMACY, TABRIZ UNIVERSITY OF MEDICAL SCIENCES, TABRIZ 51664, IRAN
 
Abstract: 

Purpose: Carotenoids are of great interest in many scientific disciplines because of their wide distribution, diverse functions and interesting properties. The present report describes a new natural source for carotenoid production.
Methods: Halorubrum sp., TBZ126, an extremely halophilic archaeon, was isolated from Urmia Lack following culture of water sample on marine agar medium and incubation at 30oC. Then single colonies were cultivated in broth media. After that the cells were collected and carotenoids were extracted with acetone-methanol (7: 3 v/v). The identification of carotenoids was performed by UV-VIS spectroscopy and confirmed by thin layer chromatography (TLC) in the presence of antimony pentachloride (SbCl5). The production profile was analyzed using liquid-chromatography mass spectroscopy (LC-MS) techniques. Phenotypic characteristics of the isolate were carried out and the 16S rRNA gene was amplified using polymerase chain reaction (PCR).
Results: LC-MS analytical results revealed that produced carotenoids are bacterioruberin, lycopene and
b-carotene. Bacterioruberin was found to be the predominant produced carotenoid.16S rRNA analysis showed that TBZ126 has 100% similarity with Halorubrum chaoviator Halo-G*T (AM048786).
Conclusion: Halorubrum sp. TBZ126, isolated from Urmia Lake has high capacity in the production of carotenoids. This extremely halophilic archaeon could be considered as a prokaryotic candidate for carotenoid production source for future studies.

 
Keyword(s): CAROTENOIDS, HALORUBRUM CHAOVIATOR, BACTERIORUBERIN, LYCOPENE, β-CAROTENE
 
 
References: 
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Click to Cite.
APA: Copy

NAZIRI, D., & HAMIDI, M., & HASSANZADEH, S., & TARHRIZ, V., & MALEKI ZANJANI, B., & NAZEMYIEH, H., & HEJAZI, M., & HEJAZI, M. (2014). ANALYSIS OF CAROTENOID PRODUCTION BY HALORUBRUM SP. TBZ126, AN EXTREMELY HALOPHILIC ARCHEON FROM URMIA LAKE. ADVANCED PHARMACEUTICAL BULLETIN, 4(1), 61-67. https://www.sid.ir/en/journal/ViewPaper.aspx?id=408342



Vancouver: Copy

NAZIRI DAVOOD, HAMIDI MASOUD, HASSANZADEH SALAR, TARHRIZ VAHIDEH, MALEKI ZANJANI BAHRAM, NAZEMYIEH HOSSEIN, HEJAZI MOHAMMD AMIN, HEJAZI MOHAMMAD SAEID. ANALYSIS OF CAROTENOID PRODUCTION BY HALORUBRUM SP. TBZ126, AN EXTREMELY HALOPHILIC ARCHEON FROM URMIA LAKE. ADVANCED PHARMACEUTICAL BULLETIN. 2014 [cited 2021May06];4(1):61-67. Available from: https://www.sid.ir/en/journal/ViewPaper.aspx?id=408342



IEEE: Copy

NAZIRI, D., HAMIDI, M., HASSANZADEH, S., TARHRIZ, V., MALEKI ZANJANI, B., NAZEMYIEH, H., HEJAZI, M., HEJAZI, M., 2014. ANALYSIS OF CAROTENOID PRODUCTION BY HALORUBRUM SP. TBZ126, AN EXTREMELY HALOPHILIC ARCHEON FROM URMIA LAKE. ADVANCED PHARMACEUTICAL BULLETIN, [online] 4(1), pp.61-67. Available: https://www.sid.ir/en/journal/ViewPaper.aspx?id=408342.



 
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