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Paper Information

Journal:   JUNDISHAPUR JOURNAL OF MICROBIOLOGY (JJM)   AUGUST 2014 , Volume 7 , Number 8; Page(s) 0 To 0.
 
Paper: 

ISOLATION AND CHARACTERIZATION OF GRAM-POSITIVE BIOSURFACTANT-PRODUCING HALOTHERMOPHILIC BACILLI FROM IRANIAN PETROLEUM RESERVOIRS

 
 
Author(s):  ZARGARI SAEED, RAMEZANI AMIN, OSTVAR SASAN, REZAEI RASOOL, NIAZI ALI*, AYATOLLAHI SHAHAB
 
* BIOTECHNOLOGY INSTITUTE, SHIRAZ UNIVERSITY, SHIRAZ, IR IRAN
 
Abstract: 

Background: Petroleum reservoirs have long been known as the hosts of extremophilic microorganisms. Some of these microorganisms are known for their potential biotechnological applications, particularly production of extra and intracellular polymers and enzymes.
Objectives: Here, 14 petroleum liquid samples from southern Iranian oil reservoirs were screened for presence of biosurfactant
?producing halothermophiles.
Materials and Methods: Mixture of the reservoir fluid samples with a minimal growth medium was incubated under an N2 atmosphere in 40°C; 0.5 mL samples were transferred from the aqueous phase to agar plates after 72 hours of incubation; 100 mL cell cultures were prepared using the MSS-1 (mineral salt solution 1) liquid medium with 5% (w/v) NaCl. The time-course samples were analyzed by recording the absorbance at 600 nm using a spectrophotometer. Incubation was carried out in 40°C with mild shaking in aerobic conditions. Thermotolerance was evaluated by growing the isolates at 40, 50, 60 and 70°C with varying NaCl concentrations of 5% and 10% (w/v). Halotolerance was evaluated using NaCl concentrations of 5%, 10%, 12.5% and 15% (w/v) and incubating them at 40°C under aerobic and anaerobic conditions. Different phenotypic characteristics were evaluated, as outlined in Bergey’s manual of determinative bacteriology. Comparing 16S rDNA sequences is one of the most powerful tools for classification of microorganisms.
Results: Among 34 isolates, 10 demonstrated biosurfactant production and growth at temperatures between 40oC and 70oC in saline media containing 5%
?15% w/v NaCl. Using partial 16S rDNA sequencing (and amplified ribosomal DNA restriction analysis [ARDRA]) and biochemical tests (API tests 20E and 50 CHB), all the 10 isolates proved to be facultative anaerobic, Gram-positive moderate thermohalophiles of the genus Bacillus (B. thermoglucosidasius, B. thermodenitrificans, B. thermoleovorans, B. stearothermophilus and B. licheniformis), exhibiting surface-active behaviors.
Conclusions: General patterns include decreasing the thermotolerance with increasing the salt concentrations and also more halotolerance in the aerobic environment compared with anaerobic conditions. The results demonstrated that Iranian petroleum reservoirs enjoy a source of indigenous extremophilic microorganisms with potential applications in microbial enhanced oil recovery and commercial enzyme production.

 
Keyword(s): BACILLUS, MICROORGANISM, PETROLEUM
 
 
References: 
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+ Click to Cite.
APA: Copy

ZARGARI, S., & RAMEZANI, A., & OSTVAR, S., & REZAEI, R., & NIAZI, A., & AYATOLLAHI, S. (2014). ISOLATION AND CHARACTERIZATION OF GRAM-POSITIVE BIOSURFACTANT-PRODUCING HALOTHERMOPHILIC BACILLI FROM IRANIAN PETROLEUM RESERVOIRS. JUNDISHAPUR JOURNAL OF MICROBIOLOGY (JJM), 7(8), 0-0. https://www.sid.ir/en/journal/ViewPaper.aspx?id=408331



Vancouver: Copy

ZARGARI SAEED, RAMEZANI AMIN, OSTVAR SASAN, REZAEI RASOOL, NIAZI ALI, AYATOLLAHI SHAHAB. ISOLATION AND CHARACTERIZATION OF GRAM-POSITIVE BIOSURFACTANT-PRODUCING HALOTHERMOPHILIC BACILLI FROM IRANIAN PETROLEUM RESERVOIRS. JUNDISHAPUR JOURNAL OF MICROBIOLOGY (JJM). 2014 [cited 2021May18];7(8):0-0. Available from: https://www.sid.ir/en/journal/ViewPaper.aspx?id=408331



IEEE: Copy

ZARGARI, S., RAMEZANI, A., OSTVAR, S., REZAEI, R., NIAZI, A., AYATOLLAHI, S., 2014. ISOLATION AND CHARACTERIZATION OF GRAM-POSITIVE BIOSURFACTANT-PRODUCING HALOTHERMOPHILIC BACILLI FROM IRANIAN PETROLEUM RESERVOIRS. JUNDISHAPUR JOURNAL OF MICROBIOLOGY (JJM), [online] 7(8), pp.0-0. Available: https://www.sid.ir/en/journal/ViewPaper.aspx?id=408331.



 
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