Click for new scientific resources and news about Corona[COVID-19]

Paper Information

Journal:   JUNDISHAPUR JOURNAL OF MICROBIOLOGY (JJM)   JUNE 2014 , Volume 7 , Number 6; Page(s) 0 To 0.
 
Paper: 

CHARACTERIZATION AND TRANSFERRING OF HUMAN ROTAVIRUS DOUBLE-LAYERED PARTICLES IN MA104 CELLS

 
 
Author(s):  TEIMOORI ALI, SOLEIMANJAHI HOORIEH*, MAKVANDI MANOOCHEHR
 
* DEPARTMENT OF VIROLOGY, FACULTY OF MEDICAL SCIENCE, TARBIAT MODARES UNIVERSITY, TEHRAN, IR IRAN
 
Abstract: 

Background: Rotavirus (RV) is a major cause of gastroenteritis in infants and children and is one of the most severe public health problems. Rotaviruses outer layer contains two proteins including VP4 and VP7. These proteins are necessary for host-cell binding and penetration. TLP (triple layer virus particle) of RV is a complete infectious virion that binds to the target cells and internalized at the cytoplasm. The DLP (double layer virus particle) is a non-infectious particle that is formed through exclusion of the outer layer proteins including VP4 and VP7. These DLPs are the transcriptionally active forms of rotavirus.
Objectives: The aim of this study was to transfer DLP of RV into cytoplasm of MA104 cells by Lipofectamine and to analyze their replication.
Materials and Methods: Initially, rotavirus was purified by CsCl discontinuous gradient and DLP was separated from TLP based on density differences. For confirmation, sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) of the proteins were conducted Then the purified DLP of RV was transferred into MA104 cells using Lipofectamine.
Results: We attempt to avoid the attachment and entry of the rotavirus by using Lipofectamine to mediate the delivery of viral particles directly into the cytoplasm. DLP was endocytosed into the cytoplasm following treatment by Lipofectamine and then replicated in cytoplasm.
Conclusions: Therefore the non-infectious DLPs were became infectious if introduced into the cytoplasm of permissive and cancerous cells, without passing attachment and entry process.

 
Keyword(s): ROTAVIRUS, TRANSFECTION, VIRAL PLAQUE ASSAY
 
 
References: 
  • ندارد
  •  
 
Click to Cite.
APA: Copy

TEIMOORI, A., & SOLEIMANJAHI, H., & MAKVANDI, M. (2014). CHARACTERIZATION AND TRANSFERRING OF HUMAN ROTAVIRUS DOUBLE-LAYERED PARTICLES IN MA104 CELLS. JUNDISHAPUR JOURNAL OF MICROBIOLOGY (JJM), 7(6), 0-0. https://www.sid.ir/en/journal/ViewPaper.aspx?id=408322



Vancouver: Copy

TEIMOORI ALI, SOLEIMANJAHI HOORIEH, MAKVANDI MANOOCHEHR. CHARACTERIZATION AND TRANSFERRING OF HUMAN ROTAVIRUS DOUBLE-LAYERED PARTICLES IN MA104 CELLS. JUNDISHAPUR JOURNAL OF MICROBIOLOGY (JJM). 2014 [cited 2021May14];7(6):0-0. Available from: https://www.sid.ir/en/journal/ViewPaper.aspx?id=408322



IEEE: Copy

TEIMOORI, A., SOLEIMANJAHI, H., MAKVANDI, M., 2014. CHARACTERIZATION AND TRANSFERRING OF HUMAN ROTAVIRUS DOUBLE-LAYERED PARTICLES IN MA104 CELLS. JUNDISHAPUR JOURNAL OF MICROBIOLOGY (JJM), [online] 7(6), pp.0-0. Available: https://www.sid.ir/en/journal/ViewPaper.aspx?id=408322.



 
  pdf-File
Yearly Visit 80
 
Latest on Blog
Enter SID Blog