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Paper Information

Journal:   JUNDISHAPUR JOURNAL OF MICROBIOLOGY (JJM)   JUNE 2014 , Volume 7 , Number 6; Page(s) 0 To 0.
 
Paper: 

OPTIMIZATION OF L-TRYPTOPHAN BIOSYNTHESIS FROM L-SERINE OF PROCESSED IRANIAN BEET AND CANE MOLASSES AND INDOLE BY INDUCED ESCHERICHIA COLI ATCC 11303 CELLS

 
 
Author(s):  SADEGHIYAN RIZI TAHEREH*, FOOLADI JAMSHID, HERAVI MAJID MOMHED, SADRAI SIMA
 
* DEPARTMENT OF PHARMACEUTICAL BIOTECHNOLOGY, FACULTY OF PHARMACY, ISFAHAN UNIVERSITY OF MEDICAL SCIENCES, ISFAHAN, IR IRAN
 
Abstract: 

Background: L-tryptophan is an important ingredient in medicines, especially in neuromedicines such as antidepressants. Many commercial processes employ various microorganisms with high tryptophan synthase activity to produce L-tryptophan from indole and L-serine, but these processes are very costly due to the costs of precursors, especially L-serine.
Objectives: For this reason, we studied the ability to use processed Iranian cane and beet molasses as L-serine sources for L-tryptophan production, which enables us to reach a cost-effective process.
Materials and Methods: Whole cells of Escherichia coli ATCC 11303 were induced for L-tryptophan synthase by addition of indole to the growth medium and bacterial cells harvested from the growth medium were used as biocatalysts in the production medium. Conditions of the production medium were optimized and Iranian cane and beet molasses were processed by solvent extraction with ethanol and n-butanol and used as L-serine sources of the production medium. Amount of L-tryptophan and theoretical yield of L-tryptophan production were determined by High Performance Liquid Chromatography and by a colorimetrical method on the basis of the remaining indole assay, respectively.
Results: L-tryptophan production increased by 15 folds, when indole was used as an inducer. L-tryptophan was produced from processed Iranian beet molasses in satisfactory amounts (0.53 mM) and no exogenous pyridoxal phosphate was required as a cofactor under our experimental conditions.
Conclusions: The obtained results proved that Iranian beet molasses include significant amounts of L-serine that makes them a suitable substitution for L-serine. Findings of the present study give impetus to use of Iranian beet molasses for cost-effective L-Trp production in the amino acid industry.

 
Keyword(s): TRYPTOPHAN, TRYPTOPHAN SYNTHASE, INDOLE
 
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