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Paper Information

Journal:   PLANT AND ECOSYSTEM   FALL 2013 , Volume 9 , Number 36-1 (SUPPLEMENT); Page(s) 113 To 126.
 
Paper: 

EVALUATING THE GENETIC DIVERSITY IN VARIOUS POPULATIONS OFBROMUS TOMENTELLUS SPECIES BASED ON STUDIES OF TOTAL PROTEIN ELECTROPHORESIS AND THEIR RELATION WITH THE ECOLOGICAL FACTORS

 
 
Author(s):  JANGALI KH.*, SALEHI P., JAFARI A.A.
 
* DEPARTMENT OF PLANT BREEDING, BORUJERD BRANCH, ISLAMIC AZAD UNIVERSITY, BORUJERD, IRAN
 
Abstract: 

The present experiment was carried out in an attempt to evaluate the genetic diversity in different populations ofBromus Tomentellus species following the studies on total protein electrophoresis and a comparison of their relation with the ecological factors. For so doing, the protein patterns of 80 genotypes from eight populations of Bromus Tomentellus species were examined to determine the degree of genetic diversity. On the basis of the results of SDS-PAGE, 24 reproducible bands, i.e. protein peptides were recorded for evaluating the genetic diversity. The percentage of polymorphism in the total bands varied from 52% to 19%. The correlation matrix of genetic and geographic distances was not proved in the Mantel test and no statistically significant correlation was found between them (P=0.400, R ^ 2=0.0049). This indicated the absence of an environmental gradient among the diversity of the total proteins. Given that Iran is the centre of various Bromus Tomentellus species, it is not surprising to observe such a high degree of diversity in various properties of this plant. Therefore, it is suggested that the Bromus Tomentellus modification programs should be extended to include diverse parents in the breeding program. A considerable genetic increase for the modification of Bromus Tomentellus can be achieved through the systematic application of germplasm which has a different protein pattern and higher qualitative properties.

 
Keyword(s): BROMUSTOMENTELLUS, GENETIC VARIATION, ELECTROPHORESIS, TOTAL PROTEIN, ECOLOGICAL FACTORS, SDS-PAGE
 
References: 
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