Paper Information

Journal:   RESEARCH IN PHARMACEUTICAL SCIENCES (RPS)   DECEMBER 2014 , Volume 9 , Number 6; Page(s) 463 To 469.
 
Paper: 

IN VITRO ANTI-HIV-1 ACTIVITIES OF KAEMPFEROL AND KAEMPFEROL-7-OGLUCOSIDE ISOLATED FROM SECURIGERA SECURIDACA

 
Author(s):  BEHBAHANI M.*, SAYEDIPOUR S., POURAZAR A., SHANEHSAZZADEH M.
 
* DEPARTMENT OF BIOTECHNOLOGY, FACULTY OF ADVANCED SCIENCES AND TECHNOLOGIES, UNIVERSITY OF ISFAHAN, ISFAHAN, I.R. IRAN
 
Abstract: 

Previously, we reported that the kaempferol and kaempferol-7-O-glucoside isolated from Securigera securidaca showed potent anti-HSV activity. In the present study the anti-HIV-1 activities of kaempferol and kaempferol-7-O-glucoside are investigated at different concentrations (100, 50, 25 and 10 ?g/ml) using HIV- 1 p24 Antigen kit. Real-time Polymerase chain reaction (RT-PCR) assay was also used for quantification of full range of virus load observed in treated and untreated cells. According to the results of RT- PCR, tested compounds at a concentration of 100 ?g/ml exerted potent inhibitory effect. Time of drug addiction experiments demonstrated that these compounds exerted their inhibitory effects on the early stage of HIV infection. The results also showed potent anti-HIV-1 reverse transcriptase activity. Antiviral activity of kaempferol-7-O-glucoside was more pronounced than that of kaempferol. These findings demonstrate that kaempferol-7-O-glucoside could be considered as a new potential drug candidate for the treatment of HIV infection which requires further assessments.

 
Keyword(s): HIV-1, ANTIVIRAL ACTIVITY, FLAVONOL, REAL TIME PCR ASSAY
 
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