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Paper Information

Journal:   JOURNAL OF ISFAHAN MEDICAL SCHOOL (I.U.M.S)   4TH WEEK SEPTEMBER 2014 , Volume 32 , Number 295; Page(s) 1 To 9.
 
Paper: 

EFFECT OF MEDIUM IN PROPERTIES AND ACTIVITY OF ACP IN THE INFECTIVE FORM OF LEISHMANIA MAJOR PROMASTIGOTES

 
 
Author(s):  NAVABI AMIR, SOLEIMANIFARD SIMINDOKHT*
 
* DEPARTMENT OF PARASITOLOGY AND MYCOLOGY, SCHOOL OF MEDICINE, ISFAHAN UNIVERSITY OF MEDICAL SCIENCES, ISFAHAN, IRAN
 
Abstract: 

Background: The wet cutaneus leishmaniasis caused by Leis hmania major infection is one of the common skin disease in many parts of the world. Reports indicate that some properties of Leis hmania such as time of infection is affected of medium. With respect to use to artificial medium in the majority of the studies on Leismania and the role of acid phosphatase in the pathogenesis of promastigotes, in this research, were studied effects of medium in properties of ACP and time of infecting in L.major promastigotes.
Methods: The present study is a cross sectional study. To culture promastigotes, L.major (MRHO/IR/75/ER) from previously infected Balb/c mice was transferred to NNN and RPMI1640 medium. Growth curve was generated and stationary stages were divided. Frozen promastigotes of each medium with sodium acetate and Triton-X-100 were homogenize and ACP was measured by calorimetric assay.
Findings: Stationary parasites were collected in NNN and RPMI-1640 medium in seventh and fifth day respectively. The rate of ACP activity was determined, 1.02
±0.08 in NNN and 1.8±0.01 in RPMI- 1640. Also Km and Vmax of this enzyme was 105.26±1.11 mM and 98.37±1.48 mM/min/mg protein in NNN and 106.39±1.14 mM and 98.04±0.96 mM/min/mg protein in RPMI1640.
Conclusion: Despite the infecting time in promastigotes, rate of agglutination, rate of kinase synthesis and virulent are different in two medium, it seems there are no significant differences in properties of acid phosphatase in stationary promastigotes in two medium.

 
Keyword(s): LEISHMANIA MAJOR, MEDIA CULTURE, ACID PHOSPHATASE, VIRULENCE
 
 
References: 
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APA: Copy

NAVABI, A., & SOLEIMANIFARD, S. (2014). EFFECT OF MEDIUM IN PROPERTIES AND ACTIVITY OF ACP IN THE INFECTIVE FORM OF LEISHMANIA MAJOR PROMASTIGOTES. JOURNAL OF ISFAHAN MEDICAL SCHOOL (I.U.M.S), 32(295), 1-9. https://www.sid.ir/en/journal/ViewPaper.aspx?id=407880



Vancouver: Copy

NAVABI AMIR, SOLEIMANIFARD SIMINDOKHT. EFFECT OF MEDIUM IN PROPERTIES AND ACTIVITY OF ACP IN THE INFECTIVE FORM OF LEISHMANIA MAJOR PROMASTIGOTES. JOURNAL OF ISFAHAN MEDICAL SCHOOL (I.U.M.S). 2014 [cited 2021May10];32(295):1-9. Available from: https://www.sid.ir/en/journal/ViewPaper.aspx?id=407880



IEEE: Copy

NAVABI, A., SOLEIMANIFARD, S., 2014. EFFECT OF MEDIUM IN PROPERTIES AND ACTIVITY OF ACP IN THE INFECTIVE FORM OF LEISHMANIA MAJOR PROMASTIGOTES. JOURNAL OF ISFAHAN MEDICAL SCHOOL (I.U.M.S), [online] 32(295), pp.1-9. Available: https://www.sid.ir/en/journal/ViewPaper.aspx?id=407880.



 
 
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