Paper Information

Journal:   JOURNAL OF ISFAHAN MEDICAL SCHOOL (I.U.M.S)   2ND WEEK SEPTEMBER 2014 , Volume 32 , Number 293; Page(s) 1 To 12.
 
Paper: 

OPTIMIZING ACRIDINE ORANGE STAINING FOR ASSESSMENT OF PROTECTIVE EFFECTS OF SAFFRON AND VITAMIN E ON RAT SPERM DNA STRUCTURE

 
 
Author(s):  RAZAVI SHAHNAZ*, VAEZ SAYYED AHMAD, MARDANI MOHAMMAD
 
* DEPARTMENT OF ANATOMICAL SCIENCES AND MOLECULAR BIOLOGY, SCHOOL OF MEDICINE, ISFAHAN UNIVERSITY OF MEDICAL SCIENCES, ISFAHAN, IRAN
 
Abstract: 

Background: In recent decades relation between ROS concentration and semen quality was noted. Saffron has traditionally been not only considered as a food additive but also as a medicinal herb, which has a good antioxidant properties. Vitamin E is considered as a endogenous and supplementary antioxidant which can protects the cell. The aim of this study was to evaluate the protection influence of saffron and vitamin E on sperm DNA against acid.
Methods: Thirty adult male Wistar rats divided into saffron (n
=10), vitamin E (n=10) and control (n=10) groups randomly. Rats received saffron (100 mg/kg/day), vitamin E (100 mg/kg/day) and distilled water (0.5 cc/day) in saffron, vitamin E and control groups respectively. After 60 days, cauda epididymis dissected and sperm were used for analysis of sperm chromatin susceptibility to acid denaturation by acridine orange (AO) staining. AO staining was carried out with or without acid detergent incubation.
Findings: Our results purposed that saffron and vitamin E can decrease sperm DNA damage against acid.
Conclusion: Saffron and vitamin E protect DNA against denaturation probably because of their powerful antioxidant properties.

 
Keyword(s): ANTIOXIDANTS, SAFFRON, VITAMIN E, DNA DAMAGE
 
References: 
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