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Paper Information

Journal:   IRANIAN JOURNAL OF APPLIED ANIMAL SCIENCE   JUN 2014 , Volume 4 , Number 2; Page(s) 425 To 428.
 
Paper: 

INVESTIGATION OF (STEAROYL-COA DESATURASE 1) SCD1 GENE POLYMORPHISM IN KHUZESTAN BUFFALO POPULATION USING PCR-RFLP METHOD

 
 
Author(s):  TAGHIZADEH K.*, BEIGI NASIRI M.T., FAYAZI J., BUJARPOOR M.
 
* DEPARTMENT OF ANIMAL SCIENCE, RAMIN AGRICULTURAL AND NATURAL RESOURCES UNIVERSITY, MOLLASANI, AHVAZ, IRAN
 
Abstract: 

Stearoyl-CoA desaturase (SCD) is a rate-limiting enzyme in the biosynthesis of monounsaturated fatty ac-ids (MUFA). A number of studies support the hypothesis that SCD gene regulation and polymorphism may affect fatty acid composition and fat quality in meat and milk. Single nucleotide polymorphisms in the cod-ing region of the bovine stearoyl-CoA desaturase gene have been predicted to result in p. 293A (alanine at amino acid 293) and p. 293V (valine at amino acid 293) alleles at the stearoyl-CoA desaturase1 locus. The objective of this study was to characterize polymorphisms of stearoyl-CoA desaturase 1 (SCD1) gene in Khuzestan buffalo population. This is the first study of SCD1 gene polymorphism in Iranian buffalo. The blood samples were taken from 85 buffalo of Khuzestan-Iran population. Genomic DNA was extracted using a kit DIAtom DNA prep. After this, PCR reactions were made by using primers that encloses exon V. A 400 bp fragment amplified with standard PCR method. The PCR products were digested by NCO1 restriction enzyme with PCR-RFLP method. All samples were genotyped on 3% agarose gels stained with ethidium bromide. Electrophoretic mobility shift determined that there were not genetic differences between these animals in the studied region by using NCOI enzyme. Also, this study showed that genotype frequencies were not in Hardy-Weinberg equilibrium for SCD1 gene.

 
Keyword(s): BUFFALO, HARDY-WEINBERG EQUILIBRIUM, MUFA, PCR-RFLP, SCD1 GENE
 
 
References: 
 
Click to Cite.
APA: Copy

TAGHIZADEH, K., & BEIGI NASIRI, M., & FAYAZI, J., & BUJARPOOR, M. (2014). INVESTIGATION OF (STEAROYL-COA DESATURASE 1) SCD1 GENE POLYMORPHISM IN KHUZESTAN BUFFALO POPULATION USING PCR-RFLP METHOD. IRANIAN JOURNAL OF APPLIED ANIMAL SCIENCE, 4(2), 425-428. https://www.sid.ir/en/journal/ViewPaper.aspx?id=407483



Vancouver: Copy

TAGHIZADEH K., BEIGI NASIRI M.T., FAYAZI J., BUJARPOOR M.. INVESTIGATION OF (STEAROYL-COA DESATURASE 1) SCD1 GENE POLYMORPHISM IN KHUZESTAN BUFFALO POPULATION USING PCR-RFLP METHOD. IRANIAN JOURNAL OF APPLIED ANIMAL SCIENCE. 2014 [cited 2021May10];4(2):425-428. Available from: https://www.sid.ir/en/journal/ViewPaper.aspx?id=407483



IEEE: Copy

TAGHIZADEH, K., BEIGI NASIRI, M., FAYAZI, J., BUJARPOOR, M., 2014. INVESTIGATION OF (STEAROYL-COA DESATURASE 1) SCD1 GENE POLYMORPHISM IN KHUZESTAN BUFFALO POPULATION USING PCR-RFLP METHOD. IRANIAN JOURNAL OF APPLIED ANIMAL SCIENCE, [online] 4(2), pp.425-428. Available: https://www.sid.ir/en/journal/ViewPaper.aspx?id=407483.



 
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