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Paper Information

Journal:   JOURNAL OF ISFAHAN MEDICAL SCHOOL (I.U.M.S)   4TH WEEK AUGUST 2014 , Volume 32 , Number 291; Page(s) 1 To 10.
 
Paper: 

UBD IDENTIFY IN PARAFFIN TISSUES IN PATIENTS WITH COLORECTAL CANCER

 
 
Author(s):  IZADI AMIR*, MOSLEMI ELHAM, POORHOSSEINI SEYED MOHAMMAD, YASSAEE VAHID REZA, KHEIRI HAMID REZA, ELIKAI HAMID REZA
 
* YOUNG RESEARCHERS AND ELITE CLUB, ISLAMIC AZAD UNIVERSITY, EAST TEHRAN BRANCH, TEHRAN, IRAN
 
Abstract: 

Background: Colon cancer is one major cause for cancer-related deaths. UBD and its alike proteins are signal transduction pathways with several cellular functions including and DNA repair control. There is relation between increase in UBD and increase in p53 expression in a way that in many cases this growth has accelerated the cancer progress. The aims of this study are to detect UBD as a marker for the early detection of colorectal cancer and disease progress in patients.
Methods: 30 samples of paraffin tissue blocks of colorectal cancer patients and 10 normal tissue samples, the selected slices were collected. RT-PCR testing to detect UBD gene and b-actin gene as an internal control were obtained.
Findings: The size of RT-PCR product of UBD gene was 395 base pair and the size of amplified bactin was 350bp. The RT-PCR of b-actin was positive in all samples. The RT-PCR products of 18 samples (95%) of male patients and 11 samples (100%) of female patients were positive. Only one sample had negative product.
Conclusion: Considering the results we can conclude that UBD as a useful marker that is independent of tumor stage plays an important role in the detection of colorectal cancer and it’s study is really important in identification and detection of early stages of disease. Results showed that the expression of the UBD in patients is 97% and it reduces to 5% in healthy people.

 
Keyword(s): UBD, COLORECTAL CANCER, RT-PCR
 
References: 
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