Paper Information

Journal:   JOURNAL OF ISFAHAN MEDICAL SCHOOL (I.U.M.S)   4TH WEEK AUGUST 2014 , Volume 32 , Number 291; Page(s) 1 To 6.
 
Paper:  BIOASSAY GUIDED FRACTIONATION OF PYCNOCYCLA SPINOSA EXTRACT USING CONTRACTION OF RAT ILEUM
 
Author(s):  SADRAEI HASAN, GHANADIAN MOSTAFA, ASGHARI GHOLAMREZA*, JAHED MASOUD
 
* ISFAHAN PHARMACEUTICAL SCIENCES RESEARCH CENTER, ISFAHAN UNIVERSITY OF MEDICAL SCIENCES, ISFAHAN, IRAN
 
Abstract: 

Background: Hydro alcoholic extract from aerial part of Pycnocycla spinosa have shown to possess antispasmodic and anti diarrhoeal activities. Anti diarrhoeal effect of the extract both quantitatively and in quality are very close to lope ramide. The aim of this research was identification of active fraction separated from extract of P. spinosa by applying bioassay method on contraction of rat ileum.
Methods: From aerial part of P. spinosa methanolic extract was prepared and then medium polar fractions were separated. The antispasmodic activities of separated fractions were assessed on rat isolated ileum contraction induced by KCl and compared with total extract. After that the most active fraction was identified for further comparison with control groups. Contraction were measured as maximum recorded amplitude and express as % of initial contraction. Full concentration response curve were plotted for each group of experiment and the IC50 value (inhibitory concentration of extract causing 50% of maximum response) was calculated.
Findings: From nine fractions isolated from P. spinosa extract, the third fraction identified as the most active fraction which contains the active substance (s). Five fractions had a medium activity and two fractions were ineffective.
Conclusion: This research demonstrated that P. spinosa possessed a number of active substances with different potencies. The most potent substance was in the third fraction and therefore, purification and identification of this substance is recommended.

 
Keyword(s): PYCNOCYCLA SPINOSA, ILEUM, EXTRACT, KCL, FRACTIONS, IC50
 
References: 
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