Click for new scientific resources and news about Corona[COVID-19]

Paper Information

Journal:   JOURNAL OF ISFAHAN MEDICAL SCHOOL (I.U.M.S)   4TH WEEK, FEBRUARY 2014 , Volume 31 , Number 266; Page(s) 2131 To 2138.
 
Paper: 

IDENTIFICATION OF NON-TUBERCULOSIS MYCOBACTERIA ISOLATES BY POLYMERASE CHAIN REACTION- RESTRICTION ENZYME ANALYSIS OF 360-BP FRAGMENT OF RPOB GENE

 
 
Author(s):  HADIFAR SHIMA, MOGHIM SHARAREH, GHASEMIAN SAFAEI HAJIEH, FAZELI HOSSEIN, FARID FARIBA, MOGHOOFEI MOHSEN, SEDIGHI MANSOUR, NASR ESFAHANI BAHRAM*
 
* DEPARTMENT OF MICROBIOLOGY, ISFAHAN UNIVERSITY OF MEDICAL SCIENCES, ISFAHAN, IRAN
 
Abstract: 

Background: Diagnosis of mycobacterium genus provides a basis for investigating the epidemiology and pathogenesis of this group of bacteria. Regarding the prevalence of mycobacterial infection in Iran and because of the being neighborhood with countries among 22 high-burden countries, increasing attention to mycobacterial diseases and introducing molecular epidemiology of Mycobacteria seems to be necessary. Restriction Fragment Length Polymorphism Analysis of Polymerase Chain Reaction-Amplified Fragments (PCR-RFLP) is an inexpensive and accurate method providing diagnosis of mycobacterial species. The present study aimed to determine the common types of Mycobacteria in this geographical region by mentioned method.
Methods: 34 clinical isolates were collected and cultured and identified by phenotypic methods. A 360-bp fragment of the rpoB gene was amplified by PCR and then, PCR products were digested by the two enzymes, MspI and HaeIII. Digested fragments were analyzed by using 4% metaphor agarose gel electrophoresis.
Findings: Out of 34 species of nontuberculous mycobacteria (NTMs), M. fortuitum type I with the frequency of 82.35% was the most frequent type and M. gordonae type I and M. kansasii type I both with the frequency of 5.88% andM. gordonae type II and M. intracellular both with the frequency of 2.94% were the next.
Conclusion: The PCR-RFLP analysis of rpoB gene used for identification of Mycobacteria provided valid results in this geographical area. In this study, M. kansasii type I (HeaIII: 90/205, MspI: 30/40/60/175) and M. avium (HeaIII: 270; MspI: 40/80/105) were identical to the patterns of some studies and different from others. This study demonstrated the high sensitivity (100%) of used PCRRFLP analysis method for identification of Mycobacteria.

 
Keyword(s): NONTUBERCULOUS MYCOBACTERIA, RESTRICTION FRAGMENT LENGTH POLYMORPHISM ANALYSIS OF POLYMERASE CHAIN REACTION-AMPLIFIED FRAGMENTS (PCR-RFLP), RPOB GENE, IDENTIFICATION
 
 
References: 
  • Not Registered.
  •  
 
+ Click to Cite.
APA: Copy

HADIFAR, S., & MOGHIM, S., & GHASEMIAN SAFAEI, H., & FAZELI, H., & FARID, F., & MOGHOOFEI, M., & SEDIGHI, M., & NASR ESFAHANI, B. (2014). IDENTIFICATION OF NON-TUBERCULOSIS MYCOBACTERIA ISOLATES BY POLYMERASE CHAIN REACTION- RESTRICTION ENZYME ANALYSIS OF 360-BP FRAGMENT OF RPOB GENE. JOURNAL OF ISFAHAN MEDICAL SCHOOL (I.U.M.S), 31(266), 2131-2138. https://www.sid.ir/en/journal/ViewPaper.aspx?id=407339



Vancouver: Copy

HADIFAR SHIMA, MOGHIM SHARAREH, GHASEMIAN SAFAEI HAJIEH, FAZELI HOSSEIN, FARID FARIBA, MOGHOOFEI MOHSEN, SEDIGHI MANSOUR, NASR ESFAHANI BAHRAM. IDENTIFICATION OF NON-TUBERCULOSIS MYCOBACTERIA ISOLATES BY POLYMERASE CHAIN REACTION- RESTRICTION ENZYME ANALYSIS OF 360-BP FRAGMENT OF RPOB GENE. JOURNAL OF ISFAHAN MEDICAL SCHOOL (I.U.M.S). 2014 [cited 2021May18];31(266):2131-2138. Available from: https://www.sid.ir/en/journal/ViewPaper.aspx?id=407339



IEEE: Copy

HADIFAR, S., MOGHIM, S., GHASEMIAN SAFAEI, H., FAZELI, H., FARID, F., MOGHOOFEI, M., SEDIGHI, M., NASR ESFAHANI, B., 2014. IDENTIFICATION OF NON-TUBERCULOSIS MYCOBACTERIA ISOLATES BY POLYMERASE CHAIN REACTION- RESTRICTION ENZYME ANALYSIS OF 360-BP FRAGMENT OF RPOB GENE. JOURNAL OF ISFAHAN MEDICAL SCHOOL (I.U.M.S), [online] 31(266), pp.2131-2138. Available: https://www.sid.ir/en/journal/ViewPaper.aspx?id=407339.



 
 
Persian Abstract Yearly Visit 34
 
Latest on Blog
Enter SID Blog