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Paper Information

Journal:   JOURNAL OF ISFAHAN MEDICAL SCHOOL (I.U.M.S)   1ST WEEK SEPTEMBER 2014 , Volume 32 , Number 292; Page(s) 1 To 10.
 
Paper: 

STUDY THE POTENTIAL OF RECOMBINANT OMP31 PROTEIN FROM BRUCELLA MELITENSIS IN INDUCING THE PROLIFERATION OF IMMUNIZED MOUSE LYMPHOCYTES IN IN VITRO

 
 
Author(s):  GHASEMI AMIR, SALARI MOHAMMAD HOSSEIN*, ZARNANI AMIR HASSAN, JEDDI TEHRANI MAHMOOD, RANJBAR REZA
 
* DEPARTMENT OF MICROBIOLOGY, SCHOOL OF PUBLIC HEALTH, TEHRAN UNIVERSITY OF MEDICAL SCIENCES, TEHRAN, IRAN
 
Abstract: 

Background: Brucella melitensis infection is still a major health problem for human and cattle in developing countries and the Middle East. Cell-mediated immunity is the dominant immune response required for protection against brucellosis. So the identification of new candidates which can induce cell-mediated immunity are demanded. Proliferation assay is one of methods is used to evaluate the potential of an antigen to generate memory Tcell. In the present study, the proliferation of speloncyets obtained from immunized mice was evaluated when they were challenged by recombinant Omp 31 (rOmp 31) in vitro.
Methods: Mice were immunized by PBS, rOmp 31 and formalin-killed B. melitensis Rev.1 with Freund’s adjuvant. At day 30 after the last immunization, spleens were removed and homogenized. Purified rOmp31 (2.5 and 5
mg/mL) was added to 2´105 spleen cell and then the speloncytes were incubated at 37 ?C in 5% CO2 atmosphere. After 48h, XTT and MTT assays were used to show the splenocyte proliferation.
Findings: The data showed that splenocytes of mice immunized with rOmp 31 or Rev.1 started to proliferate after stimulation by rOmp 31. There was a significant difference between SIs obtained from rOmp 31 and Rev groups compared to PBS groups (P
<0.01) and (P<0.05), respectively.
Conclusion: Based on our finding, rOmp 31 could generate a good memory cellular immune response. In addition, the method described here to study the speloncyte proliferation using XTT is a simple and efficient method.

 
Keyword(s): BRUCELLA, VACCINE, RECOMBINANT PROTEIN, SPLENOCYTE, PROLIFERATION ASSAY
 
 
References: 
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APA: Copy

GHASEMI, A., & SALARI, M., & ZARNANI, A., & JEDDI TEHRANI, M., & RANJBAR, R. (2014). STUDY THE POTENTIAL OF RECOMBINANT OMP31 PROTEIN FROM BRUCELLA MELITENSIS IN INDUCING THE PROLIFERATION OF IMMUNIZED MOUSE LYMPHOCYTES IN IN VITRO. JOURNAL OF ISFAHAN MEDICAL SCHOOL (I.U.M.S), 32(292), 1-10. https://www.sid.ir/en/journal/ViewPaper.aspx?id=407195



Vancouver: Copy

GHASEMI AMIR, SALARI MOHAMMAD HOSSEIN, ZARNANI AMIR HASSAN, JEDDI TEHRANI MAHMOOD, RANJBAR REZA. STUDY THE POTENTIAL OF RECOMBINANT OMP31 PROTEIN FROM BRUCELLA MELITENSIS IN INDUCING THE PROLIFERATION OF IMMUNIZED MOUSE LYMPHOCYTES IN IN VITRO. JOURNAL OF ISFAHAN MEDICAL SCHOOL (I.U.M.S). 2014 [cited 2021May18];32(292):1-10. Available from: https://www.sid.ir/en/journal/ViewPaper.aspx?id=407195



IEEE: Copy

GHASEMI, A., SALARI, M., ZARNANI, A., JEDDI TEHRANI, M., RANJBAR, R., 2014. STUDY THE POTENTIAL OF RECOMBINANT OMP31 PROTEIN FROM BRUCELLA MELITENSIS IN INDUCING THE PROLIFERATION OF IMMUNIZED MOUSE LYMPHOCYTES IN IN VITRO. JOURNAL OF ISFAHAN MEDICAL SCHOOL (I.U.M.S), [online] 32(292), pp.1-10. Available: https://www.sid.ir/en/journal/ViewPaper.aspx?id=407195.



 
 
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