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Paper Information

Journal:   JOURNAL OF GORGAN UNIVERSITY OF MEDICAL SCIENCES   FALL 2014 , Volume 16 , Number 3 (51); Page(s) 106 To 110.
 
Paper: 

APPLICATION OF PCR METHOD IN SPECIFIC IDENTIFICATION OF FUSARIUM SOLANI IN HIV POSITIVE PATIENTS

 
 
Author(s):  FERDOUSI A.*, SHAHHOSSEINY M.H., BAYAT M., HASHEMI S.J., GHAHRI M.
 
* DEPARTMENT OF PATHOBIOLOGY, ISLAMIC AZAD UNIVERSITY, SCIENCE AND RESEARCH BRACH, TEHRAN, IRAN
 
Abstract: 

Background and Objective: Fusarium solani is the common etiological agent of fungemia and disseminated fusariosis, which is associated with high incidence of mortality in immune-compromised host. Due to high level of resistance of antifungals in Fusarium solani, rapid and specific identification of organism is essential. This study was done to evaluate the PCR method for rapid and specific diagnosis of Fusarium solani in serum samples of HIV positive patients.
Methods: In this descriptive study, the PCR test based on mitochondrial cytochrome b gene as the target gene with 330 bp product was optimized. PCR was applied on 45 serum samples of HIV positive patients after evaluation of sensitivity and specificity of the test.
Results: In the optimized PCR test, the 330 bp product was amplified. The sensitivity of the test was a copy of Fusarium solani genome, and its specificity was 100%. Among 45 serum samples, 9 cases (20%) were positive for Fusarium solani.
Conclusion: The PCR method has functional capabilities for direct, rapid and specific clinical diagnosis of Fusarium solani in HIV positive patients.

 
Keyword(s): FUSARIUM SOLANI, MITOCHONDRIAL CYTOCHROME B GENE, HIV, PCR
 
References: 
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