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Paper Information

Journal:   IRANIAN ENDODONTIC JOURNAL (IEJ)   2014 , Volume 9 , Number 3; Page(s) 220 To 224.
 
Paper: 

EFFECT OF MINERAL TRIOXIDE AGGREGATE, CALCIUM-ENRICHED MIXTURE CEMENT AND MINERAL TRIOXIDE AGGREGATE WITH DISODIUM HYDROGEN PHOSPHATE ON BMP-2 PRODUCTION

 
 
Author(s):  GHASEMI NEGIN, RAHIMI SAEID*, LOTFI MEHRDAD, SOLEIMANIRAD JAFAR, SHAHI SHAHRIAR, SHAFAIE HAJAR, SALEM MILANI AMIN, SHAKUIE SAHAR, ZAND VAHID, ABDOLRAHIMI MAJID
 
* DEPARTMENT OF ENDODONTICS, DENTAL FACULTY, TABRIZ UNIVERSITY OF MEDICAL SCIENCES, TABRIZ, IRAN
 
Abstract: 

Introduction: One of the hypotheses regarding the calcification induction by mineral trioxide aggregate (MTA) is the involvement of transforming growth factor-Beta (TGF-b) super family. Calcium-enriched mixture (CEM) cement is one of the endodontic biomaterials with clinical applications similar to MTA. The aim of the present in vitro study was to compare the induction of bone morphogenic protein-2 (BMP-2) by a combination of disodium hydrogen phosphate (DSHP) and tooth colored ProRoot MTA (WMTA), to that of CEM cement and WMTA.
Methods and Materials: Human gingival fibroblasts (HGFs) were obtained from the attached gingiva of human premolars. HGFs were cultured in Dulbecco’s Modified Eagle’s medium, supplemented with 10% fetal calf serum, penicillin, and streptomycin. Cells in groups 1, 2 and 3 were exposed to WMTA, CEM and WMTA+DSHP discs, respectively. The fourth group served as the control. After 72 h of exposure, HGF viability was determined by Mosmann’s tetrazolium toxicity (MTT) assay. BMP-2 levels in cell-free culture media were determined by enzyme-linked immunosorbent assay (ELISA). Statistical analysis was performed using the one-way ANOVA, followed by the post hoc Games-Howell test for BMP-2 and post hoc Tukey’s test for the results of MTT assay.
Results: Cellular viability was significantly higher in group 3 compared to the other groups (P<0.05); however, CEM and WMTA did not exhibit significant differences (P=0.08). The control group exhibited significantly higher cellular viability in comparison to the other groups of the study (P<0.05). The highest and lowest protein production rates were observed in the WMTA (3167±274.46 pg/mL) and WMTA+DSHP (1796±839.49 pg/mL) groups, respectively. There were no significant differences between the control, WMTA and CEM groups (P>0.05).
Conclusion: WMTA and CEM did not exhibit any significant differences in terms of inducing BMP-2 production; however, incorporation of DSHP into WMTA resulted in a decrease in the induction of this protein.

 
Keyword(s): BONE MORPHOGENIC PROTEIN-2, CALCIUM-ENRICHED MIXTURE CEMENT, DISODIUM HYDROGEN PHOSPHATE, FIBROBLASTS, MINERAL TRIOXIDE AGGREGATE, TRANSFORMING GROWTH FACTOR-BETA
 
 
References: 
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Click to Cite.
APA: Copy

GHASEMI, N., & RAHIMI, S., & LOTFI, M., & SOLEIMANIRAD, J., & SHAHI, S., & SHAFAIE, H., & SALEM MILANI, A., & SHAKUIE, S., & ZAND, V., & ABDOLRAHIMI, M. (2014). EFFECT OF MINERAL TRIOXIDE AGGREGATE, CALCIUM-ENRICHED MIXTURE CEMENT AND MINERAL TRIOXIDE AGGREGATE WITH DISODIUM HYDROGEN PHOSPHATE ON BMP-2 PRODUCTION. IRANIAN ENDODONTIC JOURNAL (IEJ), 9(3), 220-224. https://www.sid.ir/en/journal/ViewPaper.aspx?id=407071



Vancouver: Copy

GHASEMI NEGIN, RAHIMI SAEID, LOTFI MEHRDAD, SOLEIMANIRAD JAFAR, SHAHI SHAHRIAR, SHAFAIE HAJAR, SALEM MILANI AMIN, SHAKUIE SAHAR, ZAND VAHID, ABDOLRAHIMI MAJID. EFFECT OF MINERAL TRIOXIDE AGGREGATE, CALCIUM-ENRICHED MIXTURE CEMENT AND MINERAL TRIOXIDE AGGREGATE WITH DISODIUM HYDROGEN PHOSPHATE ON BMP-2 PRODUCTION. IRANIAN ENDODONTIC JOURNAL (IEJ). 2014 [cited 2021May10];9(3):220-224. Available from: https://www.sid.ir/en/journal/ViewPaper.aspx?id=407071



IEEE: Copy

GHASEMI, N., RAHIMI, S., LOTFI, M., SOLEIMANIRAD, J., SHAHI, S., SHAFAIE, H., SALEM MILANI, A., SHAKUIE, S., ZAND, V., ABDOLRAHIMI, M., 2014. EFFECT OF MINERAL TRIOXIDE AGGREGATE, CALCIUM-ENRICHED MIXTURE CEMENT AND MINERAL TRIOXIDE AGGREGATE WITH DISODIUM HYDROGEN PHOSPHATE ON BMP-2 PRODUCTION. IRANIAN ENDODONTIC JOURNAL (IEJ), [online] 9(3), pp.220-224. Available: https://www.sid.ir/en/journal/ViewPaper.aspx?id=407071.



 
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