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Paper Information

Journal:   IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES   APRIL 2014 , Volume 17 , Number 4; Page(s) 256 To 262.
 
Paper: 

SPECTROFLUORIMETRIC DETERMINATION OF CEFIXIME USING TERBIUM-DANOFLOXACIN PROBE

 
 
Author(s):  MANZOORI JAMSHID L.*, AMJADI MOHAMMAD, SOLTANI NASER, JOUYBAN ABOLGHASEM
 
* DEPARTMENT OF ANALYTICAL CHEMISTRY, FACULTY OF CHEMISTRY, UNIVERSITY OF TABRIZ, TABRIZ, IRAN
 
Abstract: 

Objective (s): Cefixime (Cfx), is a semi-synthetic third-generation oral cephalosporin antibiotic that is prescribed for the treatment of susceptible infections. There are some procedures for the determination of Cfx in pharmaceutical formulations and biological samples. Herein a spectrofluorimetric method was proposed for Cfx determination based on the fluorescence quenching of terbium-danofloxacin (Tb3+-Dano) in the presence of Cfx.
Materials and Methods: Cfx was detected based on fluorescence quenching of terbium-danofloxacin (Tb3+-Dano) in the presence of Cfx with maximum excitation and emission wavelengths at 347 nm and 545 nm, respectively. The quenched fluorescence intensity of Tb3+- Dano system is proportional to the concentration of Cfx. The optimum conditions for the determination of Cfx were studied.
Results: The maximum response was achieved under optimum conditions of [Tris buffer]= 0.008 mol/l (pH 6.5), [Tb3+]=1×10-4 mol/l and [Dano]=1×10-4 mol/l. The developed method was evaluated in terms of accuracy, precision and limit of detection. The linear concentration ranges for quantification of Cfx were 8.8×10-8-8.8×10-7 mol/l and 1.1×10-7-8.8×10-7 mol/l in standard and human serum samples with the detection limits (S/N=3) of 2.8×10-8 mol/l and 3.9×10-8 mol/l, respectively. The Cfx was determined in pharmaceutical tablets and spiked serum samples and the results were satisfactory.
Conclusion: This method is simple, practical and relatively interference-free for determination of Cfx in pharmaceutical tablets and serum samples.

 
Keyword(s): CEFIXIME, DANOFLOXACIN, QUENCHING, SPECTROFLUORIMETRY, TERBIUM-SENSITIZED
 
 
References: 
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Click to Cite.
APA: Copy

MANZOORI, J., & AMJADI, M., & SOLTANI, N., & JOUYBAN, A. (2014). SPECTROFLUORIMETRIC DETERMINATION OF CEFIXIME USING TERBIUM-DANOFLOXACIN PROBE. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, 17(4), 256-262. https://www.sid.ir/en/journal/ViewPaper.aspx?id=405841



Vancouver: Copy

MANZOORI JAMSHID L., AMJADI MOHAMMAD, SOLTANI NASER, JOUYBAN ABOLGHASEM. SPECTROFLUORIMETRIC DETERMINATION OF CEFIXIME USING TERBIUM-DANOFLOXACIN PROBE. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES. 2014 [cited 2021May11];17(4):256-262. Available from: https://www.sid.ir/en/journal/ViewPaper.aspx?id=405841



IEEE: Copy

MANZOORI, J., AMJADI, M., SOLTANI, N., JOUYBAN, A., 2014. SPECTROFLUORIMETRIC DETERMINATION OF CEFIXIME USING TERBIUM-DANOFLOXACIN PROBE. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, [online] 17(4), pp.256-262. Available: https://www.sid.ir/en/journal/ViewPaper.aspx?id=405841.



 
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