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Paper Information

Journal:   IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES   DECEMBER 2013 , Volume 16 , Number 12; Page(s) 1259 To 1265.
 
Paper: 

AN EFFICIENT COVALENT COATING ON GLASS SLIDES FOR PREPARATION OF OPTICAL OLIGONUCLEOTIDE MICROARRAYS

 
 
Author(s):  POURJAHED ATEFEH, RABIEI MOHAMMAD*, TAHRIRI MOHAMMADREZA
 
* BIOMATERIALS GROUP, FACULTY OF BIOMEDICAL ENGINEERING, AMIRKABIR UNIVERSITY OF TECHNOLOGY, TEHRAN, IRAN
 
Abstract: 

Objective (s): Microarrays are potential analyzing tools for genomics and proteomics researches, which is in needed of suitable substrate for coating and also hybridization of biomolecules.
Materials and Methods: In this research, a thin film of oxidized agarose was prepared on the glass slides which previously coated with poly-L-lysine (PLL). Some of the aldehyde groups of the activated agarose linked covalently to PLL amine groups; also bound to the amino groups of biomolecules. These linkages were fixed by UV irradiation. The prepared substrates were compared to only agarose-coated and PLL-coated slides.
Results: Results on atomic force microscope (AFM) demonstrated that agarose provided three-dimensional surface which had higher loading and bindig capacity for biomolecules than PLL-coated surface which had two-dimensional surface. In addition, the signal-to-noise ratio in hybridization reactions performed on the agarose-PLL coated substrates increased two fold and four fold compared to agarose and PLL coated substrates, respectively.
Conclusion: The agarose-PLL microarrays had the highest signal (2546) and lowest background signal (205) in hybridization, suggesting that the prepared slides are suitable in analyzing wide concentration range of analytes.

 
Keyword(s): AGAROSE, COATING, MICROARRAY, PLL, SIGNAL-TO-NOISE
 
 
References: 
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Click to Cite.
APA: Copy

POURJAHED, A., & RABIEI, M., & TAHRIRI, M. (2013). AN EFFICIENT COVALENT COATING ON GLASS SLIDES FOR PREPARATION OF OPTICAL OLIGONUCLEOTIDE MICROARRAYS. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, 16(12), 1259-1265. https://www.sid.ir/en/journal/ViewPaper.aspx?id=405663



Vancouver: Copy

POURJAHED ATEFEH, RABIEI MOHAMMAD, TAHRIRI MOHAMMADREZA. AN EFFICIENT COVALENT COATING ON GLASS SLIDES FOR PREPARATION OF OPTICAL OLIGONUCLEOTIDE MICROARRAYS. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES. 2013 [cited 2021May12];16(12):1259-1265. Available from: https://www.sid.ir/en/journal/ViewPaper.aspx?id=405663



IEEE: Copy

POURJAHED, A., RABIEI, M., TAHRIRI, M., 2013. AN EFFICIENT COVALENT COATING ON GLASS SLIDES FOR PREPARATION OF OPTICAL OLIGONUCLEOTIDE MICROARRAYS. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, [online] 16(12), pp.1259-1265. Available: https://www.sid.ir/en/journal/ViewPaper.aspx?id=405663.



 
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