Paper Information

Journal:   IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES   DECEMBER 2013 , Volume 16 , Number 12; Page(s) 1276 To 1281.
 
Paper: 

PKCA AGONISTS ENHANCE THE PROTECTIVE EFFECT OF HYALURONIC ACID ON NITRIC OXIDE-INDUCED APOPTOSIS OF ARTICULAR CHONDROCYTES IN VITRO

 
 
Author(s):  ZHOU JIAN LIN*, FANG HONG SONG, PENG HAO, HU QIONG JIE, LIU SHI QING, MING JIANG HUA, QIU BO
 
* DEPARTMENT OF ORTHOPEDICS, RENMIN HOSPITAL OF WUHAN UNIVERSITY, WUHAN, 430060, HUBEI PROVINCE, PEOPLE’S REPUBLIC OF CHINA
 
Abstract: 

Objective (s): Protein kinase C (PKC?) is involved in modulating articular chondrocytes apoptosis induced by nitric oxide (NO). Hyaluronic acid (HA) inhibits nitric oxide-induced apoptosis of articular chondrocytes by protecting PKC?, but the mechanism remains unclear. The present study was performed to investigate the effects and mechanisms of PKC? regulate protective effect of hyaluronic acid.
Materials and Methods The ratio of apoptotic cell and cell viability was surveyed by PCNA and MTT assay. The expression of caspase-3 was determined by real-time PCR and western blot.
Results: It was showed that HA was able to reduce the nuclei fragment and PCNA expression, and NO-induced articular apoptosis blocked by HA, pretreated chondrocytes with PMA, HA significantly inhibits the activation of caspase-3 induced by NO, but pretrement with CHR, HA significantly incresed the expression of caspase-3.
Conclusion: The results may be showed that PKCa regulate the expresion of caspase-3, which contribute to the apoptosis of chondrocytes induced by NO. PKC ? agonists enhance the protective effect of hyaluronic acid on nitric oxide-induced articular chondrocytes apoptosis.

 
Keyword(s): APOPTOSIS, NITRIC OXIDE, PKC-α, PMA
 
References: 
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