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Paper Information

Journal:   IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES   FEBRUARY 2014 , Volume 17 , Number 2; Page(s) 81 To 86.
 
Paper: 

SIGNIFICANCE OF MICRORNA TARGETED ESTROGEN RECEPTOR IN MALE FERTILITY

 
 
Author(s):  ABHARI ALIREZA, ZARGHAMI NOSRATOLLAH, SHAHNAZI VAHIDEH, BARZEGARI ABOLFAZL, FARZADI LAYA, KARAMI HADI, ZUNUNI VAHED SEPIDEH, NOURI MOHAMMAD*
 
* WOMENS REPRODUCTIVE HEALTH RESEARCH CENTER, ALZAHRA HOSPITAL, TABRIZ, IRAN
 
Abstract: 

Objective(s): Estrogen receptor-alpha (ERa) mediates estrogen action in regulation of different levels of the hypothalamic-pituitary-testis axis. It has a key role in spermatogenesis. Estrogen receptor alpha knock-out (ER koa) male mice were infertile and severe impairment in spermatogenesis and seminiferous tubules was observed. Recently, it has been reported that microRNA (miRNA) mir-100 and let-7b were predicted to target ERa gene. MiRNA are small, endogenous, single stranded RNA molecules that regulate gene expression and have been implicated in various disease states. It has been proved that some miRNAs expression is tissue- and disease-specific, giving potential for identifying miRNAs as a diagnostic tool.
Materials and Methods: In this study, the change in the expression levels of mir-100, let-7b and ER
a expression levels were evaluated in oligospermic infertile patients (n=43) compared to control fertile subjects (n=43). After washing and separating sperms, total RNA was isolated and then cDNA was synthesized. The expression levels of mir-100 and let-7b and ERa were evaluated by real time PCR.
Results: Mir-100, let-7b levels were significantly higher than those in control group (P=0.008 and P=0.009, respectively). We have found that, ER
a level was significantly decreased in comparison with normal group (P<0.0001).
Conclusion: Changes in mir-100, let-7b and ER
a expression levels in oligospermic patients may be associated with the susceptibility and progression of infertility. The results of this study indicate that miRNA can have a key role in spermatogenesis and might have a diagnostic and prognostic value in men infertility.

 
Keyword(s): FERTILITY, HAS-MIR-100, HAS-LET-7B, MICRORNA
 
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